电针对慢性炎性痛大鼠镇痛作用及机制研究  

Analgesic Effect and Mechanism of Electroacupuncture on Rats with Chronic Inflammatory Pain

作　　者：刘盈君[1] 方芳[1] 方剑乔[1] 张静若[1] 池细绿[1] 陈华德[1] 

机构地区：[1]浙江中医药大学第三临床医学院针灸神经生物学研究室,杭州310053

出　　处：《中国中西医结合杂志》2016年第6期690-695,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81202755);浙江省自然科学基金资助项目(No.Y206754);国家中医药管理局中医药重点学科(针灸学)建设经费资助项目(No.国中医药发[2009]30号)

摘　　要：目的观察电针对慢性炎性痛大鼠镇痛作用及患侧背根神经节及脊髓背角Mas相关G蛋白偶联受体C(Mas-related G protein-coupled receptor C,Mrgpr C)的调节机制。方法健康雄性SD大鼠40只,按随机数字表法分为正常组、模型组、针刺组、电针组,每组10只。右后足底注射完全弗氏佐剂(complete Freund's adjuvant,CFA)0.1 m L建立慢性炎性痛模型,分别测量大鼠造模前及干预第1、3、5、7日及干预后患侧足缩腿阈(paw withdrawal thresholds,PWTs)。采用免疫印迹法检测大鼠干预后患侧背根神经节和脊髓背角Mrgpr C表达,采用免疫组化法检测患侧脊髓背角牛肾上腺髓质22肽(bovine adrenal medulla 22,BAM22)含量。结果与正常组同期比较,模型组各时点PWTs降低(P<0.01)。与模型组同期比较,电针组干预第5日及干预后PWTs升高(P<0.05,P<0.01)。与针刺组同期比较,电针组干预后PWTs升高(P<0.05)。与正常组比较,模型组患侧背根神经节Mrgpr C表达及患侧脊髓背角浅层BAM22阳性细胞率升高(P<0.01)。与模型组比较,电针组患侧背根神经节Mrgpr C表达及患侧脊髓背角浅层BAM22阳性细胞率升高(P<0.05)。结论电针对CFA诱导的慢性炎性痛有良好的镇痛效应,其作用机制可能与其上调患侧背根神经节Mrgpr C表达和患侧脊髓背角BAM22含量相关。Objective To observe analgesic effect of electroacupuncture（EA） on rats with chronic inflammatory pain and its regulatory mechanism on ispilateral dorsal root ganglion（DRG） and spinal dorsal horn（SDH） Mas-related G protein-coupled C receptor（MrgprC）.Methods Totally 40 healthy male SD rats were divided into 4 groups according to random number table,i.e.,the normal（N） group,the model（M）group,the acupuncture（Acu） group,the EA group,1 0 rats in each group.The model of chronic inflammatory pain was established by subcutaneous injecting 0.1 mL complete Freund＇s adjuvant（CFA） into right hind paw.Paw withdrawal thresholds（PWTs） were measured before modeling,at day 1,3,5,7,and after CFA injection,respectively.Expression levels of MrgprC in ispilateral DRG and SDH were detected by Western blot.The content of bovine adrenal medulla 22（BAM22） in SDH was detected by immunohistochemical assay.Results Compared with N group at each time point,PWTs significantly decreased in M group（P 〈0.01）.Compared with M group,PWTs significantly increased at day 5 of EA and after EA in EA group（P 〈0.05,P 〈0.01）.Compared with Acu group at each time point,post-EA PWTs significantly increased in the EA group（P 〈0.05）.Compared with N group,expression of MrgprC in ispilateral DRG and ratio of BAM22 positive cells in ispilateral SDH increased in M group（P 〈0.01）.Compared with M group,expression of MrgprC in ispilateral DRG and ratio of BAM22 positive cells in ispilateral SDH increased in the EA group（P0.05）.Conclusion EA had favorable analgesic effect on chronic inflammatory pain induced by CFA,and its mechanism might be possibly associated with up-regulating MrgprC expression in ispilateral DRG and BAM22 content in ispilateral SDH.

关 键 词：慢性炎性痛 电针 Mas相关G蛋白偶联受体C 牛肾上腺髓质22肽 背根神经节 脊髓背角 

分 类 号：R245[医药卫生—针灸推拿学]