麝香酮对脑损伤大鼠脑保护作用的研究  

Brain Protection of Muscone in Rats with Brain Injury

作　　者：姜涛[1] 黄李法[1] 周水晶[1] 崔建军[1] 叶强[1] 

机构地区：[1]浙江省中医院神经外科,杭州310018

出　　处：《中国中西医结合杂志》2016年第6期724-728,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2010ZA030)

摘　　要：目的观察麝香酮鼻腔给药对创伤性脑损伤模型大鼠脑保护作用影响。方法 SD大鼠按随机数字法分为假手术组、模型组和治疗组,每组各50只,通过控制性皮层撞击制备创伤性脑损伤模型,假手术组只进行手术及麻醉程序,无冲击损伤过程。治疗组采用大鼠在体鼻腔循环灌流法将麝香酮(1.8 mg/kg)从鼻腔给药,30 min/次,2次/日,共7日。检测各组动物干预前(T1)、干预第3日(T2)、干预第5日(T3)、干预后(T4)脑组织含水量,干预结束后采用免疫组织化学方法分析检测脑源性神经营养因子(brain derived neurotrophic factor,BDNF)和神经生长因子(nerve growth factor,NGF)表达。结果与假手术组比较,模型组T1-4脑含水量升高(P<0.05),NGF、BDNF表达降低(P<0.01)。与模型组比较,治疗组T1-3脑含水量降低(P<0.05),NGF、BDNF表达升高(P<0.01)。结论创伤性脑损伤后鼻腔给予麝香酮可降低脑含水量,减轻脑水肿,促进嗅鞘细胞分泌BDNF和NGF。Objective To observe cerebral protective effect of muscone（nasal administration） on traumatic brain injury model rats.Methods SD rats were divided into the sham-operation group,the model group,and the treatment groups according to random digit table,50 in each group.Traumatic brain injury model was established by controlled cortical strike.Rats in the sham-operation group received surgery and anesthesia procedures only,with no strike.Muscone（1.8 mg/kg） was delivered to rats in the treatment group using in situ nasal perfusion,30 min each time,twice daily for 7 successive days.Water content of brain tissue was detected in each group before intervention（T1）,at day 3 of intervention（T2）,day 5 of intervention（T3）,and after intervention（T4）,respectively.Expression levels of brain derived neurotrophic factor（BDNF） and nerve growth factor（NGF） were detected using immunohistochemical analysis.Results Compared with the sham-operated group,water content of brain tissue increased（P 〈0.05）,and expression levels of NGF and BDNF decreased in the model group at T1,T2,T3,and T4（P 〈0.01）.Compared with the model group,water content of brain tissue decreased（P0.05）,and expression levels ofNGF and BDNF increased（P0.01） in the treatment group at T1,T2,and T3.Conclusion Nasal administration of muscone could reduce water content of brain tissue,alleviate cerebral edema,promote secretion of BDNF and NGF by olfactory ensheathing cells in traumatic brain injury rats.

关 键 词：麝香酮 创伤性脑损伤 神经营养因子 神经生长因子 鼻腔给药 嗅鞘细胞 

分 类 号：R285.5[医药卫生—中药学]