植物生长调节素对西南凤尾蕨组织培养的影响  

Effects of Plant Growth Regulators on Tissue Culture of Pteris wallichianain

作　　者：王一诺[1] 韦莹[1] 李翠[1] 李林轩[1] 肖冬[1] 王晓峰[1] 韦坤华[1] 

机构地区：[1]广西壮族自治区药用植物园/广西药用资源保护与遗传改良重点实验室,南宁530023

出　　处：《湖北农业科学》2016年第8期2117-2119,共3页Hubei Agricultural Sciences

基　　金：广西自然科学基金项目(桂科自0991025Z);广西科学研究与技术开发计划项目(桂科合14125008-2-21;桂科重1355001-3-4)

摘　　要：以西南凤尾蕨(Pteris wallichiana)的成熟孢子为外植体,以MS、1/2MS为基本培养基,研究了植物生长调节素对西南凤尾蕨离体组织培养的影响。结果表明,不同细胞分裂素和生长激素配比对西南凤尾蕨的组织培养影响不同,最适诱导培养基为MS+6-BA 1.0 mg/L+NAA 2.0 mg/L;最适增殖培养基为MS+6-BA 1.0 mg/L+NAA 1.0 mg/L+AC 1.0 g/L,最高增殖倍数为7.05倍;最适生根壮苗培养基为1/2MS+6-BA 0.5 mg/L+NAA 0.5 mg/L,生根率达到90%以上。In this research, the spore of Pteris wallichianais was used as the explants and the MS,1 / 2MS were used as the basic culture medium to evaluate the effects of hormone combination on callus formation and plantlet regeneration of Pteri swallichiana. The results showed that the effects of cytokinin and anxinratio on tissue culture of Pteris wallichiana were significantly different,the best initial medium was MS＋6-BA 1.0 mg / L＋NAA 2.0 mg / L.The combination of 6-BA and NAA processed an improvement effect for the micropropagation,and the best multiplication medium was MS＋6-BA 1.0 mg / L＋NAA 1.0 mg / L＋AC 1.0 g / L,the highest proliferation times was 7.05. And the best rooting medium was 1 / 2MS ＋6-BA 0.5 mg / L ＋NAA 0.5mg / L, the rooting ratio was above 90%.

关 键 词：西南凤尾蕨(Pteris wallichiana) 植物生长调节素 组织培养 

分 类 号：S682.35[农业科学—观赏园艺]