柴达木盆地梭梭LEA基因的扩增及序列分析  

Amplification and Sequence Analysis of LEA Gene of Haloxylon ammodendron in Qaidam Basin

作　　者：马玉花[1] 冶贵生[1] 冯志鹏[1] 

机构地区：[1]青海大学农牧学院,西宁810016

出　　处：《湖北农业科学》2016年第9期2383-2385,共3页Hubei Agricultural Sciences

基　　金：青海省科技厅自然科学基金项目(2012-Z-938Q);"123高层次人才培养工程"项目

摘　　要：为发掘梭梭(Haloxylon ammodendron)抗逆相关基因,以柴达木盆地梭梭的同化枝为材料,扩增其LEA基因并进行了序列测定与分析。结果表明,所得柴达木盆地梭梭的LEA基因碱基数为234 bp,其中A碱基70个,T碱基52个,G碱基59个,C碱基53个;氨基酸序列分析表明,所得LEA基因片段编码78个氨基酸,其中强碱性氨基酸有12个,强酸性氨基酸有6个,疏水性氨基酸有34个,亲水性氨基酸有26个。The fragment of LEA of wild Haloxylon ammodendron in Qaidam Basin was amplified by the specific primers,then the nucleotide sequence and amino acid sequence of LEA of Haloxylon ammodendron was identified. The results showed that the total length of LEA gene was 234 bp, the number of A,T,G,C were 70, 52, 59 and 53 respectively. The animo acid composition analysis shows that the total length of animo acid of LEA gene was 78, and there have 12 strongly basic（＋） amino acids, 6 strongly acidic（-） amino acids, 34 hydrophobic amino acids and 26 hydrophilic amino acid.

关 键 词：梭梭(Haloxylon ammodendron) LEA基因 扩增 序列分析 

分 类 号：Q781[生物学—分子生物学]