基于LFY2int2和SSR的浙江云和梨种质鉴定  

Identification of Yunhe pears in Zhejiang province based on LFY2int2 and SSR analyses

作　　者：郑小艳[1] 杨舒贻[1] 周晓音 潘芝梅[3] 

机构地区：[1]丽水学院,浙江丽水323000 [2]丽水市农作物站,浙江丽水323000 [3]丽水职业技术学院,浙江丽水323000

出　　处：《果树学报》2016年第6期641-648,共8页Journal of Fruit Science

基　　金：浙江省自然科学基金项目(LQ13C020001);云和传统雪梨优系选育(1期;编号:2014XPZ04);丽水学院校级学生科研项目(2013年34号);丽水水果产业科技创新团队(编号:2012cxtd07)

摘　　要：【目的】明确浙江云和梨地方品种的遗传背景,为优良品种选育提供科学依据。【方法】基于低拷贝核基因LFY2int2的DNA序列和SSR对16份云和梨种质进行了分子鉴定。【结果】供试样本中共有4种LFY2int2拷贝（a,b1,b2和c）,除了‘真香梨1’（c/b2）和‘云和红梨’（b1/b2）外,其余样本均为a/b1。基于LFY2int2的系统发育树表明‘真香梨1’与所有中国砂梨（Pyrus pyrifolia）样本分开独立成支;12对SSR引物在供试样本中分别扩增出了2～6个等位基因,UPGMA聚类结果显示‘真香梨1’和‘云和红梨’分别以0.45和0.70的相似系数与其他云和梨样本分开,而其余样本可分为2组,其中‘老雪梨4’和‘薄皮雪梨2’一致,‘老雪梨3’和‘老雪梨10’一致。【结论】基于LFY2int2和SSR的分析结果表明‘真香梨1’与中国砂梨亲缘关系远,它可能是砂梨和西洋梨（P.communis）的杂交后代;‘云和红梨’与典型云和雪梨起源不同;此外鉴定出了2组可能为同物异名的种质。[Objective]Yunhe pears are a group of Chinese sand pears（Pyrus pyrifolia） native to Lishui,Zhejiang province,China and can be divided into two types,Laoxueli and Zhenxiangli,which are important and unique germplasm resources for their large fruits and/or lateness in ripening compared with the current sand pear cultivars.Some fine selections of Yunhe pears were collected and planted under unified conditions as candidates for selection and breeding.However,some of these selections showed a high level of similarity on morphological characteristics and phenological phases,suggesting the possibility of synonyms.So far,little has been known about the genetic background of Yunhe pears,especially Zhenxiangli.To provide a basis for the breeding of Yunhe pear cultivars,their genetic background and relationships among these fine selections needs to be estimated.[Methods]Eleven fine selections of Laoxueli and one Zhenxiangli accession were collected from Yunhe and Jingning in Lishui,grafted onto ＇Cuiguan＇ and grown under uniform conditions.One semi-wild accession,＇ YHHL＇,and three other Laoxueli accessions（＇XHXL＇,＇BPXL1＇ and ＇BPXL2＇） were collected from local orchards.Young leaves of these 16 accessions were collected and the total genomic DNA was extracted by using CTAB methods for the following PCRs.The second intron of the LEAFY2 gene,LFY2int2,was amplified by using primers of LFY2-F and LFY2- R.The PCR products were directly sequenced,and more than three TA clones were sequenced when there were intra-individual divergent copies.The LFY2int2 sequences of Chinese sand pears and Meili native to Zhejiang province and P.betuleaforlia obtained in our previous study were aligned with sequences obtained in this study using Clustal X.Phylogenetic trees based on Maximum Parsimony methods were constructed using MEGA6 with sequence of Malus domestica（GenBank accession number DQ535886） as an outgroup.12 SSR markers explored in previous studies were selected.NH026 a,CH02bl0,CH01bl2 and CH01f02 were geno

关 键 词：云和梨 LFY2int2 SSR 品种选育 

分 类 号：S661.2[农业科学—果树学]