一测多评法测定决明子中蒽醌苷元类和萘并吡喃酮苷类有效成分含量  

Determination of naphthopyrone glycosides and anthraquinone aglycone in Cassia tora L by quantitative analysis of multi-components by single marker

作　　者：魏喜芹[1] 魏世杰[1] 马研妮 

机构地区：[1]宁夏医科大学总医院药剂科,宁夏银川750004

出　　处：《中国生化药物杂志》2016年第4期189-192,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的建立决明子中蒽醌苷元类和萘并吡喃酮苷类有效成分含量的一测多评法。方法采用Sunfire C18色谱柱（4.6mm×250 mm,5μm）,柱温30℃,乙腈-0.1%磷酸水溶液梯度洗脱,检测波长278 nm和284 nm,测定橙黄决明素与红镰霉素龙胆二糖苷、大黄酚和大黄素甲醚间的相对校正因子,并考察其重现性,比较计算值与测得值的差异。结果红镰霉素龙胆二糖苷、橙黄决明素、大黄酚和大黄素甲醚分别在：0.0503-1.5078、0.3197-9.5899、0.5070-15.2108、0.4027-12.0814μg范围呈良好线性;回归方程分别为：Y=4.95X＋2.01（R2=0.9998）、Y=1.03X＋0.03（R2=0.9999）、Y=3.98X-0.12（R2=0.9993）、Y=4.81X＋0.26（R2=0.9996）;用一测多评法对6批决明子中大红镰霉素龙胆二糖苷、大黄酚和大黄素甲醚进行测定,与外标法实测值基本一致。结论该方法可靠,结果准确,可用于决明子的质量控制。Objective To develop a method of quantitative analysis of multi-components by single marker（ QAMS） for determination of effective components of naphthopyrone glycosides and anthraquinone aglycone in Cassia tora L. Methods The separation was performed on a Sunfire C18column（ 4. 6 mm × 250 mm,5 μm）,and column temperature 30 ℃. Aetonitrile-0. 1% H3PO4 was used as the mobile phase with gradient elution. The detection wavelength was at 278 nm and 284 nm. Relative correction factor by determination of Between aurantio-obtusin and rubrofusarin-gentiobioside or chrysophanol or physcion. the method was evaluated for reproducibility,and the difference between calculated and measured values was compared.Results Rubrofusarin-gentiobioside,aurantio-obtusin, chrysophanol and physcion respectively 0. 0503-1. 5078,0. 3197-9. 5899,0. 5070-15. 2108,0. 4027-12. 0814 μg showed a good linear relationship,and the regression equation is Y = 4. 95 X ＋ 2. 01（ R2= 0. 9998）,Y = 1. 03 X ＋ 0. 03（ R2=0. 9999）,Y = 3. 98X-0. 12（ R2= 0. 9993）,Y = 4. 81 X ＋ 0. 26（ R2= 0. 9996）. The quantitative results of six batches of Cassia tora L by QAMS was basically consistent with that by external standard method. Conclusion The QAMS method was reliable and accurate,which might be used for the quality control of Cassia tora L.

关 键 词：一测多评法 决明子 萘并吡喃酮苷 蒽醌苷元 红镰霉素龙胆二糖苷 橙黄决明素 大黄酚 大黄素甲醚 

分 类 号：R284.1[医药卫生—中药学]