Box-Behnken效应面法优化妇乐颗粒中绿原酸提取工艺  

Optimization of processing parameters for extraction of chlorogenic acid in Fule Granula using Box-Behnken experimental design

作　　者：林佳苗 姜建伟[2] 

机构地区：[1]浙江省台州恩泽医疗中心(集团)台州市中心医院,浙江台州318000 [2]浙江省肿瘤医院,浙江杭州310022

出　　处：《中国生化药物杂志》2016年第4期196-198,202,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的采用Box-Behnken效应面法,优选妇乐颗粒中绿原酸提取工艺条件。方法采用HPLC测定妇乐颗粒中绿原酸的含量,色谱柱:Agilent ZORBAX Eclipse XDB C18(4.6 mm×250 mm,5μm);检测波长327 nm;柱温:30℃;流速:0.9 m L/min;流动相∶乙腈-0.2%磷酸溶液=15∶85。以绿原酸提取率为指标,在单因素试验基础上,通过Box-Behnken试验考察提取时间、料液比、提取次数对提取工艺的影响。结果最佳提取工艺为加12倍量水提取3次,每次60 min;绿原酸提取率为3.379%。结论通过BoxBehnken效应面法为评价系统优选的提取工艺合理可行,预测性良好,为妇乐颗粒的应用提供参考。Objective To optimize the processing parameters for the extraction of chlorogenic acid in Fule Granula by Box-Behnken experimental design. Methods The content of chlorogenic acid in Fule Granula was determination. The separation was performed on a Agilent ZORBAX Eclipse XDB C18column（ 4. 6 mm × 250 mm,5 μm）,and column temperature 30 ℃. Aetonitrile-0. 2% H3PO4 was used as the mobile phase. The detection wavelength was at 327 nm. A three-factor and three-level Box-Behnken experimental design was employed to investigate effects of extraction time,solidliquid ratio and extraction times on composite score of extracting amount of chlorogenic acid. Results Optimum process conditions were as follows： 12 times the amount of water extracted three times,each time 60 min; chlorogenic acid extraction rate of 3. 379%. Conclusion This optimized extraction technology has good predictability by Box-Behnken experimental design,and overall desirability,it provides a reference for application of Fule Granula.

关 键 词：妇乐颗粒 忍冬藤 绿原酸 提取工艺 BOX-BEHNKEN效应面法 

分 类 号：R284.1[医药卫生—中药学]