GABA对热应激雏鸡法氏囊组织中IL-IL-6、TNF-TNF-α和HSPHSP70 mRNA表达的影响  

Effects of GABA on Expressions of IL-6,TNF-α and HSP70 mRNA in Bursa of Fabricius of Chicks under Heat Stress

作　　者：周永蔚 梁臣[1] 姜迎娅[1] 陈忠[1] 

机构地区：[1]海南师范大学,热带动植物生态学教育部重点实验室,海南海口571158

出　　处：《中国家禽》2016年第10期27-31,共5页China Poultry

基　　金：国家自然科学基金项目(31260555、31560680)

摘　　要：试验旨在研究γ-氨基丁酸（GABA）对1-6周龄热应激雏鸡法氏囊组织中IL-6、TNF-α、HSP70 mRNA表达的影响。选择体重相近的1日龄雄性文昌鸡108只,随机平均分为对照组（CK）、热应激（HS）组、GABA＋热应激（GABA＋HS）组,饲养6周。每周龄末剖取法氏囊组织,采用RT-PCR方法检测雏鸡法氏囊组织中IL-6、TNF-α、HSP70 mRNA的表达水平,分析其在1-6周龄不同组雏鸡法氏囊组织中表达的变化。结果显示：与CK组比较,HS组和GABA＋HS组雏鸡法氏囊组织中IL-6 mRNA表达水平上升,且GABA＋HS组法氏囊组织中IL-6 mRNA普遍低于HS组;三组的TNF-α mRNA在1-3周龄时均有较低的表达水平,在4-6周龄时表达升高;1-6周龄HS组和GABA＋HS组雏鸡法氏囊组织HSP70 mRNA表达水平整体普遍低于CK组。结果表明：热应激导致雏鸡法氏囊组织中IL-6和TNF-α mRNA的表达水平上升,同时抑制HSP70 mRNA的表达,而GABA在一定程度上能够缓解热应激造成的损伤。This experiment was conducted to observe the effects of γ-aminobutyric acid（GABA）on the expressions of IL-6,TNF-α,HSP70 mRNA in bursa of Fabricius of 1 to 6-week-age chicks under heat stress. 108 one-day-age chicks with similar body weight were randomly divided into control group（CK）,heat stress（HS）group,GABA＋heat stress（GABA＋HS）group. The chicks were raised for 6 weeks and the bursa tissues were collected at the end of week.And the expressions levels of IL-6,TNF-α,HSP70 mRNA in Bursa of 1 to 6-week-age chicks in three groups were analyzed and detected by RT-PCR. The results showed that the expression level of IL-6 mRNA in bursa of chicks in HS and GABA＋HS groups were higher than that of CK group,while IL-6 mRNA expression in GABA＋HS group was generally lower than that of HS group. There was low expression of TNF-α mRNA among the three groups at 1 to3 weeks of age,but higher at 4 to 6 weeks of age. The expression level of HSP70 mRNA in bursa of HS and GABA＋HS groups at 1 to 6 weeks of age were generally lower than that of CK group. The results suggested that heat stress caused increase of expression levels of IL-6,TNF-α mRNA,and inhibited the expression level of HSP70 mRNA. GABA could alleviate the negative effects of heat stress on bursa tissue.

关 键 词：鸡 热应激 法氏囊 细胞因子 GABA HSP70 

分 类 号：S831.5[农业科学—畜牧学]