复方双金清胆胶囊的薄层鉴别和含量测定探讨  

Study on TLC and Quantitative Determination by UV-HPLC for Fufang Shuangjinqingdan Capsules

作　　者：赵瑜卿 桂双英[1,3] 

机构地区：[1]安徽中医药大学,合肥230012 [2]芜湖市中医医院,芜湖241000 [3]安徽省中医药科学院药物制剂研究所,合肥230012

出　　处：《海峡药学》2016年第3期74-76,共3页Strait Pharmaceutical Journal

摘　　要：目的建立复方双金清胆胶囊的质量控制方法。方法采用薄层色谱法对制剂中黄芩、黄连、大黄进行定性鉴别;采用HPLC法测定制剂中绿原酸含量。结果薄层色谱中均检出黄芩、黄连、大黄,且斑点清晰,阴性均无干扰,专属性强;绿原酸在3.426~342.6μg·m L^(-1)范围内线性关系良好(r^2=0.9997),平均回收率为98.8%,RSD为1.2%(n=6)。结论本实验建立的方法简便、准确、重现性好,可用于复方双金清胆胶囊的质量控制。OBJECTIVE To establish the quality standard of Fufang Shuangjinqingdan capsules. METHODS scutellaria,coptis and rhubarb were identified by TLC. The content of chlorogenic acid was determined by UVHPLC. RESULTS The spots on TLC plates were clear without interference in the blank reference. The response of chlorogenic acid was linear in the ranges of 3. 426 - 342. 6μg · m L^-1（ r^2 = 0. 9997）,the average recovery was98. 8%,RSD = 1. 2%. CONCLUSION The method is simple,accurate,reproducible and can be used for the quality control of Qinxuan Qingyan Capsule.

关 键 词：复方双金清胆胶囊 绿原酸 薄层鉴别 高效液性色谱 

分 类 号：R927[医药卫生—药学]