植物生长物质对西洋参种子与离体种胚的休眠解除作用  

Effects of plant growth substances on dormancy breaking of seeds and embryos in vitro of American ginseng

作　　者：秦公伟[1,2] 曹小勇[1,2] 赵桦[1,2] 徐皓[1,2] 陈艳丽[2] 魏夏媛 

机构地区：[1]陕西理工学院陕西省资源生物重点实验室,陕西汉中723000 [2]陕西理工学院生物科学与工程学院,陕西汉中723000

出　　处：《陕西理工学院学报（自然科学版）》2016年第3期46-52,共7页Journal of Shananxi University of Technology:Natural Science Edition

基　　金：陕西省教育厅科学研究计划项目(12JK0847);陕西理工学院科研基金资助项目(SLGKY12-09);陕西省优势学科生物学建设经费资助项目

摘　　要：基于植物组织培养技术,以层积处理西洋参种子为材料,研究植物生长物质对其和离体种胚休眠的解除作用。结果表明:(1)酒精10 s、次氯酸钠10 min处理是试验研究中西洋参种子消毒的最佳方法;(2)植物生长物质GA_3、ZT、IAA、NAA对西洋参种子的休眠解除要比其离体种胚困难;(3)GA_3是西洋参种胚休眠解除的有效植物生长物质,而其他植物生长物质ZT、IAA、NAA主要起到壮苗作用;(4)培养基MS+2I+5G是试验研究中筛选出适合种胚萌发生长的最佳培养基,可用于无菌苗培养;(5)相同的植物生长物质作用下,分化发育较好的种胚(裂口种子种胚)更易被解除休眠。American ginseng seeds after stratification were used to investigate the effects of plant growth substances on dormancy breaking of them and their embryos in vitro based on the plant tissue culture. The results showed that：（ 1） the best disinfection method for American ginseng seeds was alcohol 10 s,sodium hypochlorite 10 min in this experiment.（ 2） dormancy breaking of american ginseng seeds were more difficulty than their embryos in vitro by plant growth substances including GA_3,ZT,IAA,and NAA.（ 3） GA_3 was the effective plant growth substance for dormancy breaking of embryos in vitro,other plant growth substances,including ZT,IAA,and NAA were minor ones.（ 4） MS ＋ 2I ＋ 5G was the best culture medium suitable for Germination and growth of American ginseng embryos in vitro to develop plantlets.（ 5） the dormancy of better developed embryos was more likely to be breaked broken by the same plant growth substances.

关 键 词：西洋参 种子 种胚 休眠 萌发 

分 类 号：S567.2[农业科学—中草药栽培]