电针对ApoE^(-/-)小鼠肝脏PPARα与TNF-α蛋白表达的影响  

Effect of Electroacupuncture on Protein Expression of PPARα and TNF-α in Liver of ApoE^(-/-) Mice

作　　者：孙蕊[1] 詹冬梅[2] 艾炳蔚[3] 

机构地区：[1]南京中医药大学第二临床医学院,江苏南京210023 [2]盐城市第三人民医院,江苏盐城224005 [3]南京中医药大学附属医院,江苏南京210029

出　　处：《安徽中医药大学学报》2016年第3期54-57,共4页Journal of Anhui University of Chinese Medicine

基　　金：江苏省"六大人才高峰"资助项目(2011-WS047)

摘　　要：目的探讨电针对载脂蛋白E基因敲除(ApoE^(-/-))小鼠肝脏过氧化物酶体增殖剂激活受体α(peroxisome proliferator-activated receptor alpha,PPARα)、肿瘤坏死因子α(tumor necrosis factor alpha,TNF-α)蛋白表达的影响。方法将30只ApoE^(-/-)小鼠随机分为模型组、药物组及电针组,每组10只。采用高脂饲料喂养14周的方法复制高脂血症模型。电针组小鼠接受单侧神门、内关、足三里、三阴交电针,药物组小鼠每日接受40 mg/kg辛伐他汀灌胃。免疫组织化学法检测肝脏中PPARα、TNF-α的表达,采用Western blot检测肝脏PPARα的蛋白表达。结果电针组小鼠肝脏中PPARα的蛋白表达水平高于模型组,但差异无统计学意义(P>0.05),肝脏TNF-α表达水平显著低于模型组(P<0.05)。与模型组比较,电针组小鼠肝细胞内小颗粒脂滴减少,肝细胞凋亡和坏死减少,且肝细胞保存相对完整。结论电针通过抑制炎性因子的表达而减少脂质沉积,治疗动脉硬化。Objective To investigate the effect of electroacupuncture on the protein expression of peroxisome proliferator-activated receptor alpha（PPARα）and tumor necrosis factor alpha（TNF-α）in the liver of ApoE^（-/-）mice.Methods A total of 30ApoE^（-/-）mice were randomly divided into model group,drug group,and electroacupuncture group,with 10 mice in each group.The model of hyperlipidemia was established by high-fat drink for 14 weeks.The mice in the electroacupuncture group were given electroacupuncture at Shenmen,Neiguan,Zusanli,and Sanyinjiao points,and those in the drug group were given simvastatin 40mg/kg per day by gavage.Immunohistochemistry was used to measure the expression of PPARαand TNF-αin the liver,and Western blot was used to measure the protein expression of PPARαin the liver.Results Compared with the model group,the electroacupuncture group showed nonsignificantly higher protein expression of PPARαin the liver（P〉0.05）and significantly lower expression of TNF-αin the liver（P〈0.05）.Compared with the model group,the electroacupuncture group had decreased small lipid droplets in the liver,reduced hepatocyte apoptosis and necrosis,and relatively complete hepatocytes.Conclusion Electroacupuncture can reduce liquid deposition through inhibiting the expression of inflammatory factors,so as to treat arteriosclerosis.

关 键 词：动脉粥样硬化 高脂血症 电针 PPARΑ TNF-Α 

分 类 号：R541.4[医药卫生—心血管疾病] R246[医药卫生—内科学]