高效液相色谱法同时测定骨疏灵颗粒中淫羊藿苷和β-蜕皮甾酮的含量  

Simultaneous Determination of Icariin and β-Ecdysterone in Gushuling Granule by High-performance Liquid Chromatography

作　　者：俞吉[1,2] 朱裕林[3] 朱瑞[2] 桑冉[3] 李宇[2] 陈卫东[2] 

机构地区：[1]安徽省淮南市朝阳医院药剂科,安徽淮南232001 [2]安徽中医药大学药学院,安徽合肥230012 [3]蚌埠医学院第一附属医院药剂科,安徽蚌埠233004

出　　处：《安徽中医药大学学报》2016年第3期75-78,共4页Journal of Anhui University of Chinese Medicine

基　　金：安徽省自然科学基金项目(1608085QH213)

摘　　要：目的采用高效液相色谱法建立同时测定骨疏灵颗粒中淫羊藿苷和β-蜕皮甾酮含量的方法。方法采用Waters BEH130C18色谱柱（250mm×4.6mm,3.5μm）;以乙腈-0.1%甲酸溶液为流动相梯度洗脱,检测波长为260nm。结果淫羊藿苷和β-蜕皮甾酮分别在0.0125-0.125mg/mL（r=0.9998）和0.005-0.15mg/mL（r=0.9996）范围内与峰面积呈良好的线性关系;平均加样回收率分别为99.69%和100.16%（n=6）,RSD分别为1.69%和1.43%（n=6）。结论该方法简便、准确、重复性好,可为骨疏灵颗粒的质量控制提供实验依据。Objective To establish the method of high-performance liquid chromatography（HPLC）for the simultaneous determination of icariin andβ-ecdysterone in Gushuling Granule.Methods HPLC was performed on a Waters BEH130C（18） column（250mm×4.6mm,3.5μm）with a mobile phase of acetonitrile-0.1%formic acid solution as the mobile phase for gradient elution at a detection wavelength of 260 nm.Results The concentrations of icariin andβ-ecdysterone showed a good linear relationship with the peak area within 0.0125-0.125mg/mL（r=0.999 8）and 0.005-0.15mg/mL（r=0.999 6）,respectively.The average recovery rates were 99.69%and 100.16%,respectively（n=6）,and the relative standard deviations were 1.69% and 1.43%,respectively（n=6）.Conclusion This method is simple,convenient,and accurate and has good repeatability,and it can provide an experimental basis for the quality control of Gushuling Granule.

关 键 词：骨疏灵颗粒 淫羊藿苷 β-蜕皮甾酮 高效液相色谱法 含量测定 

分 类 号：R284[医药卫生—中药学]