螺旋藻激酶对肾上腺素损伤内皮细胞分泌t-PA和PAI-1的影响  

Effect of Spirulina Kinase on Drenaline Injured t-PA and PAI-1 Expression in Human Umbilical Vein Endothelial Cells

作　　者：王慧杰[1] 肖云晓[1] 庞辉[1] 陈萌[1] 蒋皓[2] 

机构地区：[1]广西医科大学,南宁530021 [2]清华大学,北京100084

出　　处：《基因组学与应用生物学》2016年第5期1049-1053,共5页Genomics and Applied Biology

基　　金：广西壮族自治区自然科学基金(2013GXNSFAA019176)资助

摘　　要：研究不同浓度螺旋藻激酶(spirulina kinase,SPK)对肾上腺素(Adr)损伤血管内皮细胞分泌组织型纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制剂(PAI-1)的影响。采用不同浓度SPK处理人脐静脉内皮细胞(HUVEC),MTT法检测不同浓度SPK对HUVEC活力影响;建立Adr损伤模型,酶联免疫吸附法检测细胞培养液中t-PA和PAI-1含量的变化。结果显示SPK浓度在10μg/mL^2 mg/mL范围内,细胞活力明显升高且不影响细胞分泌t-PA和PAI-1的量;SPK作用于Adr损伤模型,各剂量组均可降低Adr损伤下HUVEC分泌PAI-1的量,提高t-PA/PAI-1比值。本研究揭示了SPK可通过降低PAI-1、提高t-PA/PAI-1比值来实现保护Adr损伤的血管内皮细胞。To investigate the different concentrations of Spirulina kinase（SPK） effect on drenaline injured expression tissue plasminogen activator（t-PA） and Plasminogen activator inhibitor-1（PAI-1） in human umbilical vein endothelial cells. Human umbilical vein endothelial cells（HUVEC） was incubated by different concentrations of SPK, the survival ratio of HUVEC with MTT assay; The injury model established with Adr, the varitation of t-PA and PAI-1 was measured by ELISA method. The activity of HUVEC was increased and the contents of t-PA and PAI-1 were no diference at 10 μg/m L-2 mg/m L of centration of SPK; Different concentrations of SPK can increase t-PA/PAI-1 ratio and reduce release of PAI-1 of HUVEC form adr injury. This work measured SPK can protect the HUVEC form adr injury and the possible mechanism may refer to its reduce release of PAI-1 and increase t-PA /PAI-1 ratio.

关 键 词：螺旋藻激酶 组织型纤溶酶原激活物 人脐静脉内皮细胞 纤溶酶原激活物抑制剂 肾上腺素 

分 类 号：R96[医药卫生—药理学]