芍药苷对实验性大鼠脓毒症肝损伤的保护作用  

Protective Effects of Paeoniflorin on Acute Liver Injury in Experimental Rats with Sepsis

作　　者：周丽芳[1] 彭慧云[1] 欧阳文献[1] 李双杰[1] 

机构地区：[1]南华大学儿科学院暨湖南省儿童医院肝病中心,湖南长沙410007

出　　处：《医学临床研究》2016年第5期861-864,共4页Journal of Clinical Research

摘　　要：【目的】探讨芍药苷（paeoniflorin，PF）对实验性大鼠脓毒症急性肝损伤（acuteliveriNury，ALI）的保护作用。【方法】采用盲肠结扎穿孔（Cecalligationandpuncture，CLP）术诱导制作脓毒症大鼠急性肝损伤模型，将36只SD雄性大鼠随机分为假手术组、模型组、PF干预组，每组12只。假手术组仅行开腹关腹术，模型组予CLP术，PF干预组大鼠在CLP术后立即予PF腹腔注射。观察造模处理后48h内各组大鼠的生存情况，绘制生存曲线；检测各组血清谷丙转氨酶（ALT）水平；采用黄嘌呤氧化酶法检测超氧化物歧化酶（s0D）活力，采用硫代巴比妥酸法检测丙二醛（MDA）含量；HE染色观察肝脏组织病理变化。【结果】假手术组生存率为100％，显著高于模型组生存率16．7％和PF干预组生存率66．7％（P〈0．05），而干预组生存率显著高于模型组（P〈0．05）；与假手术组比较，模型组大鼠血清ALT水平显著升高，差异有统计学意义（P〈0．05）；与模型组比较，PF干预组大鼠ALT血清水平降低，差异有统计学意义（Pd0．05）。与假手术组比较，模型组大鼠肝组织中MDA含量增加和SOD活性降低（P〈0．05）；与模型组比较，PF干预组MDA含量降低，SOD活性增强，差异均具有统计学意义（P〈0．05）。病理切片显示假手术组大鼠肝脏结构无明显改变，模型组肝细胞呈点灶状坏死、大量炎症细胞浸润，PF干预组病理损伤情况均较模型组明显改善。【结论IPF对CLP诱导的大鼠脓毒症Au具有一定的保护作用，其机制可能是抗氧化反应。[Objective]To investigate the protective effects of paeoniflorin （PF） on acute liver injury （ALI） in experi- mental rats with sepsis.[Methods]Cecal ligation puncture （CLP） was used to induce acute liver injury in rats with sepsis, 36 male SD rats were randomly divided into sham operation group, model group and PF intervention group, with 12 rats in each group. The sham operation group underwent only open abdominal surgery, the model group was given CLP, the PF intervention group received PF intraperitoneal injection immediately after CLP. 48 h after mold processing, the surviv- al situation of rats in each group was observed, the survival curve was drawn. Serum alanine aminotransferase levels were detected in each group~ xanthine oxidase method was used to detect superoxide dismutase （SOD） activity. The content of malondialdehyde （MDA） was detected by using the method of the content of MDA; HE staining was used to observe the pathological changes of liver tissue.[Results]The survival rate of the sham operated group was 100%, significantly higher than that in the model group and 66.7% in the PF intervention group （ P 〈0.05）, while the survival rate of the interven- tion group was significantly higher than that in the model group （ P 〈0.05） ; Compared with the sham operation group, the serum ALT level of the model group was significantly higher, and the difference was statistically significant （ P〈 0.05） ; Compared with the model group, the serum level of PF in the ALT intervention group was decreased, and the difference was statistically significant （P 〈 0.05）. Compared with the sham operation group, the content of MDA and the activity of SOD were decreased in the model group （ P 〈0.05） ; Compared with the model group, the content of MDA in the PF intervention group was decreased, and the SOD activity was enhanced, and the difference was statistically signifi- cant （ P 〈0.05）. Pathological sections showed no significant changes in liver structure of sham oper

关 键 词：芍药甙/药理学 脓毒症/并发症 肝/损伤 肝疾病/病因学 疾病模型 动物 

分 类 号：R631.1[医药卫生—外科学]