不同产地郁金茎叶挥发油化学成分及指纹图谱对比研究  

Comparison of Chemical Composition and GC-MS Fingerprint of Volatile Oil of Stems and Leaves of Curcumae Radix from Different Origins

作　　者：陆树萍[1] 翁金月 陈茜茜[3] 张春椿[3] 胡建荣 王家员[4] 金利思 

机构地区：[1]嘉兴市中医医院,浙江嘉兴314001 [2]温州市中心医院,浙江温州325000 [3]浙江中医药大学,浙江杭州310053 [4]温州市中医院,浙江温州325000

出　　处：《中华中医药学刊》2016年第6期1340-1344,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药优秀青年人才基金项目(2014ZQ024)

摘　　要：目的:研究不同产地郁金茎叶的GC-MS指纹图谱,以期为郁金茎叶的质量标准建立提供依据。方法:通过水蒸气蒸馏法提取10个不同产地郁金茎叶中挥发油成分,运用GC-MS分析10个产地郁金茎叶挥发油成分的组成与含量差异,建立指纹图谱,所得图谱采用"中药色谱指纹图谱相似度评价系统A版"软件进行分析并计算相似度。结果:10个批次郁金茎叶挥发油指纹图谱有11个共有峰,其中参照峰为桉叶油醇。除了四川产黄丝郁金、广西产桂郁金、乐清虹桥2号、瑞安湖岭产温郁金茎叶指纹图谱相似度<0.9,浙江瑞安马屿、乐清白石、瑞安陶山、永嘉乌牛、乐清虹桥1号、丽水云和6个产地温郁金茎叶指纹图谱相似度均>0.9。结论:不同品种的郁金茎叶挥发油成分差异较大,不同产地的温郁金茎叶挥发油成分指纹图谱也存在差异。Objective： In order to provide the basis for the establishment of quality standard of the stem and leave of Curcumae Radix,this paper researched GC-MS fingerprint of the stems and leaves of Curcumae Radix from different origins. Methods： Extract the constituents of volatile oil from 10 different origins in the stems and leaves of Curcumae Radix by steam distillation extraction method. Analyze the differences in composition and content of the 10 different origins of the stems and leaves of Curcumae Radix by GC-MS and calculate the similarity of the atlas by Chromatographic Fingerprint Similarity Evaluation System A Edition software. Results： The fingerprints of the stem and leave of Curcumae Radix from the 10 groups had 11 common peaks with reference to the peak of eucalyptus. The similarity of the fingerprints of Rui＇an Mayu,Yueqing Baishi,Rui＇an Taoshan,Yongjia Wuniu,Yueqing Hongqiao No. 1,and Lishui Yunhe was above0. 90. The similarity of the fingerprint of Sichuan,Guangxi,Yueqing Hongqiao No. 2 and Rui＇an Huling was less than0. 90. Conclusion： Different varieties of Curcumae Radix have much difference in the composition of volatile oil of the stems and leaves and there is also a difference in the fingerprints of composition of volatile oil of the stems and leaves of Curcuma wenyujin Y. H. Chen et C. Ling from different origins.

关 键 词：温郁金 茎叶 挥发油 GC-MS 指纹图谱 相似度 

分 类 号：R284.1[医药卫生—中药学]