桑叶多糖对DN大鼠的保护作用及机制研究  

Protective Effects and Mechanism of Mulberry Leaf Polysaccharide on Diabetic Nephropathy Rats

作　　者：张雨薇[1] 薛继婷[2] 李齐[2] 韩智学[2] 刘洪凤[2] 

机构地区：[1]黑龙江中医药大学基础医学院,黑龙江哈尔滨150040 [2]牡丹江医学院基础医学院,黑龙江牡丹江157011

出　　处：《中华中医药学刊》2016年第6期1381-1383,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：黑龙江省教育厅科学技术研究项目(12531739)

摘　　要：目的:探讨桑叶多糖(mulberry leaf polysaccharide,MLP)对糖尿病肾病(Diabetic nephropathy,DN)大鼠的保护作用。方法:经筛选后将Wistar大鼠随机分为正常对照组、模型组、MLP低、中、高剂量治疗组。采用一次性腹腔注射链脲佐菌素(STZ)的方法建立DN大鼠模型。给予不同剂量MLP治疗8W后,分别检测大鼠空腹血糖(FPG)、尿液中微量白蛋白(m Alb)含量、血清中尿素氮(BUN)、肌酐(Cr)含量;应用免疫组化法检测肾脏转化生长因子TGF-β1蛋白的表达、RT-PCR法检测IRS-1 mRNA的表达。结果:MLP能显著降低DN大鼠FPG、m Alb、BUN、Cr含量(P<0.01);MLP能明显上调DN大鼠肝组织IRS-1 mRNA表达(P<0.01),亦能下调DN大鼠肾脏TGF-β1蛋白的表达(P<0.01)。结论:MLP对DN大鼠的肾脏具有一定肾保护作用,其作用机制可能是通过上调DN大鼠肝组织IRS-1 mRNA表达及下调TGF-β1蛋白表达来实现。Objective： To explore the protection effect of mulberry leaf polysaccharide（ MLP） on diabetic nephropathy rats by observing the impact on insulin receptor substrate 1（ IRS1） and transforming growth factor β1（ TGF-β1） expression in DN rats kidney and liver. Methods： The Wistar rats were randomly divided into normal control group,model group,low,middle and high doses of MLP groups. DN rat model was established by intraperitoneally injecting streptozotocin. The DN rats were treated with different doses of MLP. 8 weeks later,FPG level,the contents of m Alb and the contents of BUN and Cr were determined. TGF-β1 expression of DN rats kidney was detected with immunohistochemical method and IRS-1 mRNA expression of DN rats liver with RT-PCR method. Results： MLP could significantly reduce the contents of FPG,m Alb,BUN and Cr of DN rats（ P〈0. 01）. MLP could obviously increase the IRS-1 mRNA expression in DN rats liver（ P〈0. 01） and MLP also could lower the TGF-β1 expression in DN rats kidney（ P〈0. 01）. Conclusion： MLP has certain protective effect on DN rats kidney and its mechanisms may be contributing to reducing the protein expression of TGF-β1 and increasing IRS-1 mRNA expression.

关 键 词：桑叶多糖 糖尿病肾病 转化生长因子Β1 胰岛素受体底物-1 

分 类 号：R285.5[医药卫生—中药学]