HPLC测定清热止咳颗粒中的贝母素甲和贝母素乙  

Determination of peimine and peiminine in Qingre Zhike granules by HPLC

作　　者：陈健 

机构地区：[1]重庆市涪陵食品药品检验所,重庆408000

出　　处：《华西药学杂志》2016年第3期305-306,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的 采用HPLC法测定清热止咳颗粒中贝母素甲和贝母素乙的含量。方法 色谱柱为Agilent Eclipse XDB C18（250mm×4.6 mm,5μm）,流动相为乙腈-水-二乙胺（70∶30∶0.03）,流速1.0 m L·min-1,柱温30℃,ELSD漂移管温度为70℃,增益为4。结果 贝母素甲的线性范围为0.906～4.53μg（r=0.9994）,加样回收率为98.93%,RSD=0.59%（n=6）;贝母素乙的线性范围为0.817～4.085μg（r=0.9993）,加样回收率为98.28%,RSD=0.97%（n=6）。结论 所用方法简便、准确、快速,重复性好,适用于清热止咳颗粒的质量控制。OBJECTIVE To establish an HPLC method for the determination of peimine and peiminine in Qingre Zhike granules.METHODS The Agilent Eclipse XDB C18column（250 mm × 4.6 mm,5 μm） was used with acetonitrile-water-diethylemine（70∶30∶0.03） as the mobile phase.The flow rate was 1.0 m L·min-1with the column temperature of 30 ℃.ELSD drift tube temperature was 70 ℃,and the gain value was four.RESULTS The sample of peimine in the range of 0.906-4.53 μg showed a good linear relation with the peak area（r = 0.9994）.The recovery was 98.93% with the RSD of 0.59%（n = 6）.The sample of peiminine in the range of 0.817-4.085 μg showed a good linear relation with the peak area（r = 0.9993）.The recovery was 98.28% with the RSD of 0.97%（n = 6）.CONCLUSION The method is simple,rapid and accurate with good repeatability,and suitable for the quality control of Qingre Zhike granules.

关 键 词：贝母素甲 贝母素乙 清热止咳颗粒 高效液相色谱法 蒸发光散射 含量 质量控制 

分 类 号：R917[医药卫生—药物分析学]