分光光度法测定不同品种帕栗嘎及其代用品中的马兜铃总酸  

Determination of total aristolochic acid in the different varieties of Pa Li Ga and its substitutes by UV spectrophotometry

作　　者：周则[1] 才让南加[1] 卓玛 张浩[3] 

机构地区：[1]青海大学藏医学院,青海西宁810001 [2]青海省藏医院,青海西宁810007 [3]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2016年第3期315-316,共2页West China Journal of Pharmaceutical Sciences

基　　金：青海省自然科学基金项目(2013-Z-939Q);青海大学中青年科研基金项目(2013-QYY-14);青海大学医学院藏医药校企协同科技创新基金项目

摘　　要：目的 测定4种常用藏药帕栗嘎品种（藏马兜铃、Aristolochia sp.、小木通和绣球藤）中马兜铃总酸的含量。方法 采用紫外分光光度法,检测波长390 nm。结果 马兜铃酸A 1～40μg·m L-1与吸光度有良好的线性关系（r=0.9996）,平均加样回收率为89.58%,RSD=2.29%。4个帕栗嘎品种中马兜铃总酸的含量不同,其中,藏马兜铃中马兜铃总酸的含量最高,为0.802%,Aristolochia sp.、绣球藤中的含量分别为0.237%、0.0347%,小木通中的含量最低,为0.0304%。结论 从各品种帕栗嘎中马兜铃总酸的含量及其不良反应考虑,在临床治疗中应减少藏马兜铃的应用,可用功效相同而马兜铃总酸含量较低的Aristolochia sp.、绣球藤和小木通代替。OBJECTIVE To determine aristolochic acid A in 4 varieties of Tibetan medicine Pa Li Ga.METHODS The samples were analyzed by UV spectrophotometry and detected at the wave length of 390 nm.RESULTS The calibration curve was linear（r =0.9996） within the range of 1-40 μg·m L-1for aristolochic acid A.The average recovery was 89.58% with the RSD of 2.29%.The contents of total aristolochic acid was 0.802% in Aristolochia griffithii,which was the highest,and 0.237% and 0.0347% in Aristolochia sp.and Clematis montana.The contents of total aristolochic acid in Clematis armandi Franch was the lowest（0.0304%）.CONCLUSION According to contents and toxicity of Pa Li Ga in all varieties of aristolochic acid A,The application of Aristolochia griffithii should be reduced,instead of Aristolochia sp.,Clematis montana and Clematis armandi.

关 键 词：藏药 帕栗嘎 藏马兜铃 ARISTOLOCHIA sp. 小木通 绣球藤 马兜铃总酸 紫外分光光度法 含量 

分 类 号：R917[医药卫生—药物分析学]