基于催眠药效筛选考察参苓颗粒的提取纯化工艺  

Investigation of Extraction and Purification Process of Shenling Granules Based on Screening of Hypnotic Efficacy

作　　者：程刚[1] 王丹[1] 胡奎[1] 苏梦[1] 李媛媛[1] 许汉林[1] 

机构地区：[1]湖北中医药大学药学院,武汉430065

出　　处：《中国实验方剂学杂志》2016年第12期17-22,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部科技基础专项(2014FY111100-2)

摘　　要：目的:通过主要催眠药效研究对参苓颗粒提取纯化工艺进行筛选,为确定该制剂的制备工艺提供实验依据。方法:自制单提油醇沉、单提油不醇沉、合提油醇沉及合提油不醇沉4组不同工艺路线的参苓颗粒浸膏,采用翻正反射睡眠试验考察各组浸膏对小鼠阈下、阈剂量戊巴比妥钠协同作用的影响;采用环磷酰胺、氢化可的松致阴血亏虚及水平台法剥夺睡眠48 h,建立阴血亏虚失眠证候大鼠复合模型,通过HPLC测定各组浸膏大鼠脑组织中氨基酸类神经递质谷氨酸(Glu),甘氨酸(Gly),牛磺酸(Tau)及γ-氨基丁酸(GABA)的含量变化。结果:4组浸膏对小鼠阈下、阈剂量戊巴比妥钠均表现不同程度的协同作用,尤以单提油醇沉组显著缩短小鼠睡眠潜伏期、显著延长小鼠睡眠时间;与空白组比较,模型组大鼠脑组织Glu,Tau及GABA含量均明显升高,Glu/(Tau+GABA)比例增高,经各浸膏组干预后含量均不同程度下降,其中单提油醇沉组表现更为显著,Glu/(Tau+GABA)比例也在下调。结论:参苓颗粒的制备工艺优选单独提取挥发油合并醇沉的纯化工艺路线。Objective： To provide experimental basis for guiding preparation process of Shenling granules by investigating hypnotic efficacy on extraction and purification process. Method： Four groups of extract with different process were prepared. Effects of each extract on synergistic action of pentobarbital sodium with subthreshold dose and threshold dose on mice were investigated by righting reflex test. Multiple factors model were established with Yin and blood-deficiency induced by cyclophosphamide and hydrocortisone,and sleep deprivation48 h induced by water platform method. Content levels of amino acid neurotransmitters in brain tissue of rats were determined by HPLC,glutamate（ Glu）,glycine（ Gly）,taurine（ Tau） and γ-aminobutyric acid（ GABA）,to name a few. Result： Four groups of extract had different synergistic effect on pentobarbital sodium with subthreshold dose and threshold dose on mice, especially the group of single volatile oil extraction and alcohol precipitation can significantly shorten sleep latency of mice and prolong sleeping time of mice. Compared with the blank group,contents of Glu,Tau,GABA and ratio of them in brain tissue of the insomnia model rats showed a considerable elevation. While contents of these indicators decreased in different degree after treatment of extract,among them the group of single volatile oil extraction and alcohol precipitation showed more significant,ratio of Glu /（ Tau ＋ GABA） was reduced simultaneously. Conclusion： Process route of single volatile oil extraction and alcohol precipitation is the best preparation process of Shenling granules.

关 键 词：参苓颗粒 戊巴比妥钠 阴虚 失眠 氨基酸类神经递质 

分 类 号：R283.6[医药卫生—中药学] R285.5[医药卫生—中医学]