水通道蛋白AQP5对小鼠骨髓间充质干细胞分化能力的影响  

Role of aquaporin-5 in the differentiation of bone marrow-derived mesenchymal stem cells of mice

作　　者：羿菲[1] 张莹[1] 李小曼[1] 白宁[1] 刘汀[1] 孙威[1] 曹流[1] 

机构地区：[1]中国医科大学转化医学研究院教育部医学细胞生物学重点实验室,辽宁沈阳110122

出　　处：《解剖科学进展》2016年第3期310-314,共5页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81502414);教育部"创新团队发展计划"(RT13101)

摘　　要：目的探寻水通道蛋白5(Aquaporin 5,AQP5)对小鼠骨髓间充质干细胞分化能力的影响。方法采用RT-PCR和Western Blot方法检测AQP5在小鼠骨髓间充质干细胞(BMSCs)中的表达。定向诱导BMSCs后分别进行油红O染色(成脂),茜素红S和碱性磷酸酶染色(成骨),Collagen II和番红素O染色(成软骨)。检测钙离子、碱性磷酸酶和胞外糖胺多糖含量,Real-time PCR检测成脂、成骨和成软骨分化后的特异标记分子的相对+/+m RNA表达。结果 RT-PCR和Western Blot结果发现野生型(AQP5)BMSCs中有AQP5阳性条带。油红O、茜素红S、碱-/-性磷酸酶,Collagen II和番红素O染色阳性染色率在AQP5敲除型(AQP5)BMSCs明显高于野生型。Real-time PCR结果证-/-实AQP5 BMSCs在成脂、成骨和成软骨分化特异标记分子的相对m RNA表达水平显著提高。结论小鼠BMSCs中有AQP5表达,AQP5敲除显著提高BMSCs的成脂、成骨和成软骨分化能力。Objective To investigate the effect of aquaporin 5（AQP5） on the differentiation capacity of bone marrow mesenchymal stem cells（BMSCs） of mice. Methods The expression of AQP5 in mouse BMSCs was conducted by RT-PCR and Western blot. The multiple differentiation capacity was revealed by representative staining by Oil Red O（adipogenesis）, Alizarin Red S and alkaline phosphatase（ALP）（osteogenesis） and type II collagen and Safranin O（chondrogenesis）. The contents of Ca2＋, ALP and GAG were determined. The adipogenesis and osteogenesis and chondrogenesis markers m RNAs were analyzed by Real-time PCR. Results We found that AQP5 was expressed in mouse BMSCs. The multiple differentiation capacity was significantly higher in AQP5-/- than in AQP5＋/＋ BMSCs as revealed by Oil Red O, Alizarin Red S, ALP, type II collagen and Safranin O. The relative expression levels of adipogenesis and osteogenesis and chondrogenesis markers mR NAs were increased in AQP5-/- than in AQP5＋/＋ BMSCs. Conclusion AQP5 is expressed in mouse BMSCs. The differentiation capacity of BMSCs in AQP5 deficiency was increased.

关 键 词：AQP5 骨髓起源间充质干细胞 分化潜能 小鼠 

分 类 号：Q756[生物学—分子生物学]