一氧化氮对增强UV-B辐射后白菜叶绿素荧光特性和Rubisco活化酶的影响  

Effects of Nitric Oxide and Enhanced UV-B Radiation on ChlorophyⅡFluorescence and Rubisco Activase of Chinese Cabbage Seedling

作　　者：马晓丽[1] 冀瑞萍[1] 李亚莉[2] 

机构地区：[1]晋中学院生物科学与技术学院,山西晋中030600 [2]山西省农业科学院生物技术研究中心,山西太原030006

出　　处：《核农学报》2016年第7期1440-1445,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31501772);山西省高等学校科技创新项目(20101129)

摘　　要：一氧化氮(NO)在调节植物生长发育和响应胁迫方面起着很重要的作用。为探讨NO对白菜经增强UV-B辐射后的修复作用,以中白60白菜为材料,外源喷施NO、模拟增强UV-B辐射及两者复合进行处理,研究白菜幼苗生长发育、叶绿素荧光特性、Rubisco活化酶(RCA)含量、活性及其编码基因表达量的变化。结果表明,增强UV-B辐射后白菜幼苗生长受到抑制,表现为幼苗生物量、叶片数、叶长都大幅降低,叶绿素含量减少,光合作用强度降低,叶绿素荧光特性均减弱,RCA含量、活性及其编码基因表达量均下降。而NO+增强UV-B复合处理后,受到抑制的白菜幼苗生长得到一定程度的缓解,生物量、叶片数、叶长显著提高,叶绿素含量增多,光合作用强度、叶绿素荧光特性、RCA含量、活性及其编码基因表达量均显著上升。综上,NO可以增加叶绿素含量、植株生物量,减少胁迫造成的损害,提高幼苗光合作用强度,促进增强UV-B胁迫下白菜幼苗的生长,从而防止由于增强UV-B导致白菜产量减少所引起的农业问题。本研究结果为进一步探究一氧化氮气体分子在植物抗逆生理过程中的作用提供了理论依据。Nitric oxide（ NO） plays an important role in the regulation of plant growth and response to stress. To further study the response mechanism of nitric oxide gas signal to enhanced UV-B in Chinese cabbage,we studied the changes of biomass,leaf number,leaf length,content of chlorophyll, photosynthetic rate, chlorophy Ⅱ fluorescence characteristics,content of Rubisco activase（ RCA） and its activase,its coding gene expression on 20- day age of Chinese cabbage seedling under exogenouns NO,enhanced UV-B（ 10. 8 kJ·m^-2·d^-1） radiation and combined process.The results showed that,compared with control,after enhanced UV-B radiation,biomass,leaf number,leaf length,content of chlorophyll,photosynthetic rate,and chlorophyⅡ fluorescence characteristics,rubisco activase content and its coding gene expression quantity decreased in Chinese cabbage seedling. But exogenous NO can alleviate the damage which caused by enhanced UV-B radiation on Chinese cabbage seedling. Such as,biomass,leaf number,leaf length,chlorophyⅡ fluorescence characteristics and rubisco activase content and its gene expression increased. In conclusion,NO could reduce the damage and protect the cabbage seedlings under enhanced UV-B radiation stress by means of raising the photosynthesis rate and increasing the biomass and chlorophyll content. This study provide a theoretical basis for further study on the role of nitric oxide gas in the physiological process of plant resistance.

关 键 词：白菜 一氧化氮 增强UV-B辐射 叶绿素荧光 RUBISCO活化酶 

分 类 号：S634[农业科学—蔬菜学]