自身免疫性肝炎患者外周血T细胞PD-1的表达及与肝功能指标的相关性  

Expression of PD-1 on Peripheral Blood T Cells and the Correlation with Liver Function Indicators in Patients with Autoimmune Hepatitis

作　　者：熊克宫[1] 陈丽芳[1] 魏大海[1] 孔金峰[1] 孙芳[1] 林太杰[1] 柯坤宇[1] 

机构地区：[1]福建省福州市传染病医院肝病科十一区,福州350001

出　　处：《海峡药学》2016年第2期242-245,共4页Strait Pharmaceutical Journal

基　　金：福州市卫生系统科技计划项目(2014-S-w21)

摘　　要：目的研究自身免疫性肝炎(AIH)患者外周血CD4+、CD8+T细胞表面程序性死亡受体1(PD-1)的表达,并探讨其与肝功能指标的相关性。方法收集43例AIH患者(包括活动期31例和缓解期12例),并选择18例健康体检者为对照组。采用流式细胞术检测外周血CD4+、CD8+T细胞表达PD-1的细胞百分比。结果 AIH组外周血CD4+、CD8+T细胞表达PD-1的细胞百分比(分别为7.36±4.45%、6.71±3.84%)显著高于对照组(分别为3.78±2.32%、3.47±2.18%)(P均<0.05);AIH活动期组外周血CD4+、CD8+T细胞表达PD-1的细胞百分比(分别为8.73±6.81%、7.44±2.75%)明显高于缓解期组(分别为5.42±2.14%、4.82±2.04%)和对照组(分别为3.78±2.32%、3.47±2.18%)(P均<0.05),且缓解期组高于对照组(P<0.05)。AIH患者外周血CD4+、CD8+T细胞PD-1表达与TBIL、ALT、AST及Ig G水平均成正相关关系(r分别为0.781、0.887、0.825、0.893,P均<0.05)。结论 AIH患者外周血CD4+、CD8+T细胞表面PD-1表达上调,且与肝功能指标成正相关,提示PD-1可能在AIH的发生及发展中发挥重要作用。OBJECTIVE To investigate the percentage of programmed cell death receptor 1（ PD-1） on peripheral blood CD4＋,CD8＋T cells and explore the correlation with liver liver function indicators in patients with autoimmune hepatitis（ AIH）. METHODS The percentages of PD-1 on CD4＋,CD8＋T lymphocytesin peripheral blood of patients with AIH（ n = 43） and healthy controls（ n = 18） was determined by flow cytometry. RESULTS We found that the percentages of PD-1 on CD4＋,CD8＋T lymphocytesin peripheral blood of patients with AIH（ 7. 36 ± 4. 45%,6. 71 ± 3. 84%,respectively） were higher than the healthy controls（ 3. 78 ± 2. 32%,3. 47 ± 2. 18%,respectively）（ all P 0. 05）. The percentages of PD-1 on CD4＋,CD8＋T lymphocytes peripheral blood in activity stage（ 8. 73 ±6. 81%,7. 44 ± 2. 75%,respectively）,remission stage（ 5. 42 ± 2. 14%,4. 82 ± 2. 04%,respectively） and the control group（ 3. 78 ± 2. 32%,3. 47 ± 2. 18%,respectively） were gradually decline. Correlation analysis showed there was significant correlation between the proportion of peripheral blood CD4＋,CD8＋T cells expressed PD-1 in AIH and TBIL,ALT,AST,Ig G levels. CONCLUSION The PD-1 expression on peripheral blood T cells is significantly higher in patients with AIH,which positively related to liver function indicators,and it may play an important role in the pathogenesis of AIH.

关 键 词：自身免疫性肝炎 程序性死亡受体1 T细胞 肝功能指标 

分 类 号：R969.4[医药卫生—药理学]