黄、渤海小黄鱼耳石元素指纹分析及其在种群补充群体识别中的应用  

Insights into population structure of juvenile small yellow croaker(Larimichthys polyactis)in the Yellow Sea and the Bohai Sea from otolith elemental fingerprints

作　　者：王玉堃[1,2] 黄建生[1] 戴芳群[1] 唐学玺[2] 孙耀[1] 金显仕[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《海洋学报》2016年第6期32-40,共9页

基　　金：国家重点基础研究发展计划项目(2015CB453300;2010CB428900)

摘　　要：利用电感耦合等离子质谱ICP-MS元素分析方法,测定了黄、渤海125尾小黄鱼的耳石元素指纹图谱,共检测到Mg、Al、Mn、Cu、Zn、Sr、Ba、Ca等8种指纹元素。不同采样站位小黄鱼耳石元素含量均存在显著性差异,线性判别分析可以有效识别不同站位的小黄鱼群体,判别成功率为65%～96%,整体判别成功率为86%。基于耳石元素指纹图谱特征进行聚类分析,可以将黄、渤海小黄鱼早期补充群体划分为渤海种群、黄海中部种群和南黄海种群,其中黄海中部种群站位交叉明显。Elemental fingerprints were measured on otolith of 125 Larimichthys polyactis juveniles collected from 5important fishery regions in the Bohai Sea and the Yellow Sea by inductively coupled plasma mass spectrometry（ICP-MS）.Otolith elemental figerprints were used as natural tags to discriminate among different sampling regions and the observed site-specific elemental differences in small yellow croaker otoliths suggest a high level of site-fidelity in relation to the 5fishery regions.Classification accuracy rates from linear discriminate function analyses（LDFA）of otolith chemistry data were high for each region with a mean accuracy of 86%,ranging from 65%-96%,considering every possible combination of elements.Furthermore,cluster analysis of multi-elemental tags suggest that the populations of juvenile fish could be divided into three groups,the Bohai group,the Central Yellow Sea group and the South Yellow Sea group.

关 键 词：耳石 微化学 小黄鱼 群体识别 种群结构 元素指纹图谱 

分 类 号：S917.4[农业科学—水产科学]