Proteomics Analysis of Up-regulated NPM1 Protein in Colorectal Cancer  

作　　者：WANG Hai HE Chengyan YANG Zhaowei GAO Shen LI Lingxia SUN Xiaoying FANG Ling LIU Ning LI Hongjun 

机构地区：[1]Jilin University China-Japan Union Hospital, Changchun 130033, P. R. China [2]The Seeond Hospital of Jilin University, Changehun 130041, P. R. China

出　　处：《Chemical Research in Chinese Universities》2016年第3期402-405,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China（Nos.81572082, 81472030, 20973074）, the Science Founda tion of Jilin Provincial Science＆Technology Department, China（No.2011713, 20130413017GH, 20150414015GH）, the Health and Family Planning Commission of Jilin Province, China（No.2015Z041） and the Scientific Support Project from Bethune Department of Jilin University, China（Nos.2012210, 2013207051）.

摘　　要：In order to identify potential protein targets involved in colorectal cancer（CRC）, we used a liquid chro- matography coupled with mass spectrometry（LC-MS）/MS-based proteomics approach to characterize global protein expression patterns in malignant tissues and their adjacent healthy tissues from Dukes C stage CRC patients. A total number of 34 differentially expressed proteins were detected and identified by LC-MS/MS and database searching, which are supposed to be relevant to progression of colorectal tumor. Among these proteins, nucleophosmin I（NPM1） was found to be remarkably up-regulated in colorectal carcinoma tissues, as compared with that in their normal counterparts. The results presented here could provide clues to elucidate the pathological significance of NPM1 in regulation of carcinogenesis of Dukes C stage colorectal tumors.In order to identify potential protein targets involved in colorectal cancer（CRC）, we used a liquid chro- matography coupled with mass spectrometry（LC-MS）/MS-based proteomics approach to characterize global protein expression patterns in malignant tissues and their adjacent healthy tissues from Dukes C stage CRC patients. A total number of 34 differentially expressed proteins were detected and identified by LC-MS/MS and database searching, which are supposed to be relevant to progression of colorectal tumor. Among these proteins, nucleophosmin I（NPM1） was found to be remarkably up-regulated in colorectal carcinoma tissues, as compared with that in their normal counterparts. The results presented here could provide clues to elucidate the pathological significance of NPM1 in regulation of carcinogenesis of Dukes C stage colorectal tumors.

关 键 词：Liquid chromatography-mass spectrometry（LC-MS） Colorectal cancer（CRC） Nucleophosmin I（NPM1） 

分 类 号：Q51[生物学—生物化学] O657.63[理学—分析化学]