减肥胶囊中维生素BT的毛细管电泳法测定  

Determination of Vitamin BT in Slimming Capsules by Capillary Electrophoresis

作　　者：于海霞[1] 周慧[1] 赵文燕[2] 张红芬[2] 陈安家[2] 

机构地区：[1]山西医科大学细胞生理学省部共建教育部重点实验室,山西太原030001 [2]山西医科大学药学院,山西太原030001

出　　处：《中国医药工业杂志》2016年第6期786-788,共3页Chinese Journal of Pharmaceuticals

基　　金：山西医科大学创新基金(01201305)

摘　　要：采用毛细管电泳-间接紫外法测定减肥胶囊中的维生素BT。以4 mmol/L奎宁甲醇溶液作为背景吸收物质,以20mmol/L磷酸二氢钠溶液（p H 2.7）作为运行缓冲溶液,运行电压10 k V,检测波长218 nm。维生素BT在1~40μg/ml范围内线性关系良好,回收率为99.6%,RSD为1.9%。A capillary electrophoresis method was established for the determination of vitamin BT in slimming capsules with indirect UV detection. The method was carried out with 4 mmol/L quinine methanol solution as the absorbable matrix, 20 mmol/L sodium dihydrogen phosphate solution （pH 2.7） as the running buffer, at the operating voltage of 10 kV and the detection wavelength of 218 nm. It was linear for vitamin BT in the range of 1--40 μg/ml. The average recovery was 99.6 %, with RSD of 1.9 %.

关 键 词：维生素BT 间接紫外法 毛细管电泳 测定 

分 类 号：O657.8[理学—分析化学]