胆固醇酯转移蛋白基因TaqIB多态性与新疆维吾尔族心房颤动患者的相关性研究  

The correlation on polymorphism of cholesterol ester transfer protein gene TaqIB and atrial fibrillation Uyghur patients in Xinjiang

作　　者：郁秀琴[1] 木胡牙提[1] 杨玉春[1] 刘志强[1] 

机构地区：[1]新疆医科大学第一附属医院综合心脏内科,乌鲁木齐830054

出　　处：《新疆医科大学学报》2016年第7期845-848,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81260036)

摘　　要：目的探讨胆固醇酯转运蛋白基因（CTEP TaqIB）T-aqIB多态性与新疆地区维吾尔族心房颤动（房颤）患者的关系。方法选取2014年2月—2015年10月新疆医科大学第一附属医院综合心脏内科收住的维吾尔族非瓣膜性房颤患者100例（房颤组）及在体检中心进行体检的维吾尔族非房颤患者100例（对照组）,从外周静脉血中提取基因组DNA,采用聚合酶链反应-限制性片段长度多态性法（PCR-PFLP）进行基因多态性检测,比较两组人群基因多态性分布的差异。结果房颤组与对照组样本CTEP TaqIB基因多态性基因型符合哈迪-温伯格（Hardy-weinberg）平衡（房颤组χ2=0.72,P=0.39;对照组χ2=0.64,P=0.42）。CTEP TaqIB基因位点的测序结果显示其存在GG型、GA型、AA型3种基因型。基因型GG、GA、AA在维吾尔族房颤人群中分别有39例（39%）、50例（50%）、11例（11%）,在对照组人群中分别有24例（24%）、54例（54%）、22例（22%）,两组基因型和等位基因分布差异有统计学意义（χ2=6.57,P=0.037 4;χ2=6.91,P=0.008 5）。结论新疆地区维吾尔族心房颤动的发生与CTEP TaqIB基因多态性有相关性。Objective To investigate the association between polymorphism of cholesterol ester transfer protein gene（CTEP）TaqIB and atrial fibrillation（AF）Uyghur patients in Xinjiang.Methods 100 cases Uyghur patients with atrial fibrillation（AF group）and 100 cases Uyghur patients with non-atrial fibrillation（control group）admitted by Heart Center of the First Affiliated Hospital of Xinjiang Medical University from February 2014 to October 2015 were selected to extract DNA samples from the peripheral blood to monitor gene polymorphism of TaqIB by polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP）technique and to compare polymorphism distribution between the two groups.Results CTEP TaqIB gene polymorphisms genotypes in AF group and control group samples was in the balance of Hardy-Weinberg equilibrium（AF groupχ2=0.72,P =0.39;control groupχ2=0.64,P =0.42）,CTEP TaqIB loci sequencing results showed the existence of GG,GA and AA genotypes.GG,GA and AA in Uighur with AF were 39cases（39%）,50cases（50%）and 11cases（11%）,while that in the control group were 24cases（24%）,54cases（54%）and 22cases（22%）,in which genotype and allele distribution differences of the two groups were statistically significant（χ2=6.574,P =0.037 4;χ2=6.91,P =0.008 5）.Conclusion TaqIB polymorphism had a quite significant correlation to the atrial fibrillation Uyghur patients in Xinjiang.

关 键 词：心房颤动 民族 CTEP 基因多态性 

分 类 号：R541.7[医药卫生—心血管疾病]