Microarray analysis of New Green Cocoon associated genes in silkworm, Bombyx mori  

作　　者：Ya-Ru Lu Song-Zhen He Xiao-Ling Tong Min-Jin Han Chun-Lin Li Zhi-Quan Li Fang-Yin Dai 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China [2]KeyLaboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University,Chongqing 400715, China

出　　处：《Insect Science》2016年第3期386-395,共10页昆虫科学（英文版）

基　　金：Acknowledgments This research was supported by the Hi-Tech Research and Development 863 Program of China （No. 2013AA102507）, National Natural Science Foundation of China （No. 31372379 and No. 31472153）, Fundamental Research Funds for the Central Universities in China （No. XDJK2013A001 and No. XDJK2014C046） and the National Undergraduate Training Programs for Innovation and Entrepreneurship （No. 201310635008）.

摘　　要：Green cocoons in silkworm, Bombyx mori, are caused by flavonoids accumulation in the silk proteins, fibroin and sericin. Despite the economic value of natural green cocoon and medical value of flavonoids, there is limited understanding of the molecular mechanism regulating flavonoids uptake in silkworm, which is tightly associated with the trait of green cocoon. The purpose of this study is to perform a comprehensive analysis to understand the molecular mechanisms of flavonoids uptake in silkworm based on microarray analyses. The study subject was the New Green Cocoon from the silkworm strains, G200 and N100, a new spontaneous dominant green cocoon trait identified in the 2000s. The genes regulating this trait are independent of other green cocoon genes previously reported. Genome-wide gene expression was compared between the New Green Cocoon producing silkworm strains, G200 and NI00, and the control sample, which is the white cocoon producing strain 872B. Among these strains, N100 and 872B are near-isogenic lines. The results showed that 130 genes have consistently changing expression patterns in the green cocoon strains when compared with the white cocoon strain. Among these, we focused on the genes related to flavonoids metabolism and absorption, such as sugar trans- porter genes and UDP-glucosyltransferase genes. Based on our findings, we propose the potential mechanisms for flavonoids absorption and metabolism in silkworm. Our results imply that silkworm might be used as an underlying model for flavonoids in pharmaceutical research.Green cocoons in silkworm, Bombyx mori, are caused by flavonoids accumulation in the silk proteins, fibroin and sericin. Despite the economic value of natural green cocoon and medical value of flavonoids, there is limited understanding of the molecular mechanism regulating flavonoids uptake in silkworm, which is tightly associated with the trait of green cocoon. The purpose of this study is to perform a comprehensive analysis to understand the molecular mechanisms of flavonoids uptake in silkworm based on microarray analyses. The study subject was the New Green Cocoon from the silkworm strains, G200 and N100, a new spontaneous dominant green cocoon trait identified in the 2000s. The genes regulating this trait are independent of other green cocoon genes previously reported. Genome-wide gene expression was compared between the New Green Cocoon producing silkworm strains, G200 and NI00, and the control sample, which is the white cocoon producing strain 872B. Among these strains, N100 and 872B are near-isogenic lines. The results showed that 130 genes have consistently changing expression patterns in the green cocoon strains when compared with the white cocoon strain. Among these, we focused on the genes related to flavonoids metabolism and absorption, such as sugar trans- porter genes and UDP-glucosyltransferase genes. Based on our findings, we propose the potential mechanisms for flavonoids absorption and metabolism in silkworm. Our results imply that silkworm might be used as an underlying model for flavonoids in pharmaceutical research.

关 键 词：FLAVONOIDS MICROARRAY New Green Cocoon SILKWORM 

分 类 号：Q78[生物学—分子生物学] S882.2[农业科学—特种经济动物饲养]