Identification and characterization of toxins in the venom gland of the Chinese bird spider, Haplopelma hainanum, by transcriptomic analysis  

作　　者：Ting-Cai Cheng Ren-Wen Long Yu-Qian Wu You-Bing Guo Duo-Lian Liu Li Peng Dai-Qin Li Dai-Wen Yang Xin Xu Feng-Xiang Liu Qing-You Xia 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China [2]Departmentof Biological Sciences, National University of Singapore, Singapore, Singapore [3]College of Life Sciences, Hunan Normal University,Changsha, China [4]College of Life Sciences, Hubei University, Wuhan, China

出　　处：《Insect Science》2016年第3期487-499,共13页昆虫科学（英文版）

摘　　要：Tarantula venoms provide a model system for studying toxin selectivity, structure-activity relationships and molecular evolution ofpeptide toxins. Previous studies have identified a large number of peptide toxins in the venom of the Chinese bird spider Haplopelma hainanum, generally regarded as a highly venomous spider. However, the lack of available RNA-seq transcriptomic and genomic data is an obstacle to understanding its venom at the molecular level. In this study, we investigated the venom gland transcriptome of/-/, hainanum by RNA-seq, in the absence of an available genomic sequence. We identi- fied 201 potential toxins among 57 18 l de novo assembled transcripts, including knottins, Kunitz-type toxins, enzymes and other proteins. We systematically identified most of the knottins and Kunitz-type toxins, some of which showed strongly biased expression in the venom gland, including members of the huwentoxin-1, huwentoxin-2 and magi-1 families. We also discovered several novel potential toxins. These data demonstrate the high molec- ular and structural diversity in the venom toxins ofH. hainanum. This study offers a useful strategy for exploring the complex components of spider venoms.Tarantula venoms provide a model system for studying toxin selectivity, structure-activity relationships and molecular evolution ofpeptide toxins. Previous studies have identified a large number of peptide toxins in the venom of the Chinese bird spider Haplopelma hainanum, generally regarded as a highly venomous spider. However, the lack of available RNA-seq transcriptomic and genomic data is an obstacle to understanding its venom at the molecular level. In this study, we investigated the venom gland transcriptome of/-/, hainanum by RNA-seq, in the absence of an available genomic sequence. We identi- fied 201 potential toxins among 57 18 l de novo assembled transcripts, including knottins, Kunitz-type toxins, enzymes and other proteins. We systematically identified most of the knottins and Kunitz-type toxins, some of which showed strongly biased expression in the venom gland, including members of the huwentoxin-1, huwentoxin-2 and magi-1 families. We also discovered several novel potential toxins. These data demonstrate the high molec- ular and structural diversity in the venom toxins ofH. hainanum. This study offers a useful strategy for exploring the complex components of spider venoms.

关 键 词：diversity Haplopelma hainanum tarantula venom TOXIN transcriptome 

分 类 号：Q949.329.5[生物学—植物学] Q959.226.2