蓬子菜中香叶木苷抗急性血瘀作用及机制研究  

Mechanism and influence on acute blood stasis syndrome of diosmin abstracted from Galium verum

作　　者：董坤[1] 寇韩旭 宁馨[1] 孙超[1] 马英丽 

机构地区：[1]黑龙江中医药大学药物化学实验室,哈尔滨黑龙江150040

出　　处：《药物评价研究》2016年第2期207-210,共4页Drug Evaluation Research

基　　金：国家自然科学基金项目(201281274034);教育部博士点博导类基金项目资助(20112327110006)

摘　　要：目的研究蓬子菜活性成分香叶木苷抗急性血瘀作用及其作用机制。方法采用sc盐酸肾上腺素复合冰水浴制备大鼠急性血瘀模型,ig给予不同剂量香叶木苷(45、90、180 mg/kg),阿司匹林为阳性药,应用血液流变测试仪、全自动血沉仪检测血液流变学指标:全血黏度、血浆黏度、红细胞比容、血沉及红细胞聚集指数;采用试剂盒法检测血浆炎症因子C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)水平。结果与模型组比较,香叶木苷能不同程度降低全血黏度、血浆黏度、红细胞比容、血沉及红细胞聚集指数,明显改善血瘀大鼠血液流变学指标;显著降低炎症因子CRP、TNF-α和IL-1β水平。结论香叶木苷有效改善急性血瘀大鼠血液流变学异常,减少炎症因子释放,从而治疗急性血瘀及静脉炎症,改善微循环。Objective To study the influence of diosmin which is one active component abstracted from Galium verum on blood rheology of rat model and investigate the mechanism. Methods Apply epinephrin sc injection and ice water bath to make rat model of blood stasis syndrome. Rats were administered with diosmin （45, 90, and 180 mg/kg） and aspirin as a positive drug. Then make a study that how diosmin ameliorated the hemorheology by assaying the indexes of blood rheology and tetrachoric including whole blood viscosity, plasma viscosity, hematocrit, erythrocyte sedimentation rate and erythrocyte aggregation index using hemeorheology instrument and automatic ESR analyzer. Meantime, we made an approach to the subject that the influence of diosmin on the relationship between blood stasis and inflammation by assaying CRP, TNF-α, and IL-1 β. Results Diosmin can improve the indexes of hemorheology obviously. It also can reduce the content of inflammatory factors. Conclution Diosmin can improve the abnormality of hemorheology, and further, it processes the properties of curing inflammation of vein and improving microcirculation by reducing the discharge of inflammatory factors effectively,

关 键 词：蓬子菜 香叶木苷 急性血瘀 静脉炎症 血液流变学 炎症因子 

分 类 号：R965[医药卫生—药理学]