加味逍遥提取物超高效液相色谱指纹图谱研究  

UPLC fingerprint of extract of Modified Xiaoyao

作　　者：李明月[1] 周立红[2] 章顺楠[2] 叶正良[3] 柳文媛[1] 

机构地区：[1]中国药科大学药物分析教研室,江苏南京210009 [2]天士力制药集团股份有限公司,创新中药关键技术国家重点实验室,天津300410 [3]天津天士力之骄药业有限公司,天津300400

出　　处：《药物评价研究》2016年第2期230-236,共7页Drug Evaluation Research

摘　　要：目的研究并建立加味逍遥提取物(柴胡、栀子、丹皮、白芍、当归、白术、茯苓、甘草、薄荷和干姜)的超高效液相色谱指纹图谱,为加味逍遥提取物质量控制提供简便、可靠方法。方法采用超高效液相色谱法,以UPLCTM HSS T3色谱柱(100 mm×2.1 mm,1.8μm)为分析柱,乙腈-0.05%磷酸水溶液为流动相,梯度洗脱,柱温30℃,体积流量为0.3 m L/min,检测波长238 nm。结果加味逍遥提取物中多数峰可以达到基线分离。用中药色谱指纹图谱相似度评价系统2012年版对10批样品的指纹图谱进行峰匹配,确定34个共有峰,10批加味逍遥提取物中共有峰的相对保留时间RSD值小于1%,指纹图谱的相似度均在0.97以上,选取共有峰在单味药材中除茯苓、柴胡、白术外,均找到归属。结论该法建立的指纹图谱稳定性好,灵敏度高,可作为加味逍遥提取物的质量控制方法,并为其复杂成分的研究提供参考和依据。Objective To provide a sensitive method for evaluation of extract of Modified Xiaoyao （Bupleuri Radix, Cape jasmine, Paeonol, Paeoniae, Alba Radix, Angelicae Sinesis Radix, Atractylodis Macrocephalae Rhizoma, Poria, Glycyrrhizae Radix et Rhizoma, Menthae haplocalycis Herba, and Zingiberis Rhizoma Praeparatum） by UPLC fingerprint. Methods UPLC was performed on UPLCTM HSS T3 column （100 mm × 2.1 mm, 1.8 μm）. The mobile phase was a gradient of acetonitrile-0.05 % phosphoric acid. The detection wavelength was set at 238 nm. The flow rate was 0.3 mL/min and column temperature was 30 ℃. Results Most peaks could be separated preferably, and the RSD values of the relative relation time of 10 batches of extract of Modified Xiaoyao were lower than 1%, 34 common peaks were obtained in the fingerprint of 10 batches of extract of Modified Xiaoyao with the help of peaks match with Similarity Evaluation System for Chromatographic Fingerprint of TCM. The similarity among fingerprints was calculated more than 0.97. The common peaks selected belonged to all the ingredients that could be used to classify the species, but Poria, Bupleuri Radix and Atractylodis Macrocephalae Rhizoma did not share common peaks. Conclusion The fingerprint could be used as a method of quality control and provides evidence for the research of effective components of Xiaoyao Powder.

关 键 词：加味逍遥提取物 超高效液相色谱 指纹图谱 

分 类 号：R917[医药卫生—药物分析学]