UItrastructural Observation on Rat Spermatozoa Treatedwith Tripchlorolide(T_4) from Tripterygium wilfordii  

雷公藤单体Y_4对大白鼠精子作用的超微结构观察

作　　者：党连凯[1] 王英[1] 戴景峰 邱劲松 张树林[1] 付广礼 孙亦彬 

机构地区：[1]中国科学院生物物理所,国家计划生育委员会科研所

出　　处：《Journal of Chinese Pharmaceutical Sciences》1995年第4期205-211,共7页中国药学（英文版）

摘　　要：Adult male rats were treated orally with monomer T_4(Tripchlorolide) isolated from Tripterygium wilfordii, at the dose of 50μg/kg/day,6 days/week for6 weeks.Ultrathin section and freeze etching replica of seminiferous tubules and epididymal spermatozoa were examined with elec-tron microscope. The results showed that the spemiogenesis was inhibited T_4 in seminiferous tu-bules. However,the damage and disruption of the spermatozoa were more serious in the epididymis.Damage of the structure and function ot microtubule and microfilament may be the chief reason for sperm damage. Sperm membrane was also very sensitive to the treatiment of monomer T_4.雄性成熟大白鼠经口喂雷公藤单体＆＿４５０μｇ／ｋｇ／日，每周６天，６周后断头处死，采用电子显微镜观察睾丸曲细精管内和附睾内的精子，结果显示Ｔ＿４影响曲细精管内精子形成过程，经过附睾后精子损伤加重。微管与微丝的结构和功能的破坏是导致精子畸形的主要原因，精子膜结构对Ｔ＿４作用也非常敏感。

关 键 词：Tripterygium wilfordii Tripchlorolide(T_4) Rat SPERMATOZOA ULTRASTRUCTURE FREEZE-ETCHING 

分 类 号：R285.5[医药卫生—中药学]