载银壳聚糖接枝香草醛胶乳的制备及抗菌性  

Preparation and the antibacterial activity of silver-loaded Chitosan-grafted-vanillin sol

作　　者：安静[1] 郭桂真[1] 罗青枝[1] 李雪艳[1] 殷蓉[1] 王德松[1] 

机构地区：[1]河北科技大学理学院,河北石家庄050018

出　　处：《河北科技大学学报》2016年第4期358-364,共7页Journal of Hebei University of Science and Technology

基　　金：国家自然科学基金(51203042);河北省自然科学基金(E2016208030)

摘　　要：为了增强壳聚糖的抗菌活性和拓展其应用领域,在其基体中引入了纳米银粒子,并对其进行了接枝改性。采用微波辐射法将香草醛与壳聚糖接枝,然后用香草醛还原硝酸银,得到均匀分散于壳聚糖香草醛接枝物(CS-g-VA)胶乳中的纳米银粒子,即Ag/CS-g-VA复合胶乳。探讨了制备Ag/CS-g-VA复合胶乳的影响因素,并采用SEM,TEM,FTIR,XRD,UV-vis和TG-DSC等对其进行了结构和热性能表征。结果表明合成Ag/CS-g-VA的较理想条件:香草醛的质量分数为6.0%,CS-g-VA的质量分数为4.0%,硝酸银的浓度为0.20 mol/L。通过抗菌测试研究了Ag/CS-g-VA对大肠杆菌和枯草杆菌的抑制作用,结果显示Ag/CS-g-VA复合胶乳具有比单一壳聚糖抗菌剂更高效的抗菌性能。In order to enhance the antibacterial activity of chitosan and expand its application field,silver nanoparticles are introduced into the matrix,and the grafting modification is carried out.Chitosan grafted vanillin(CS-g-VA)is prepared with microwave-radiation method,and silver nitrate is reduced by vanillin,so that silver nanoparticles are separated evenly in CS-g-VA sol,which is Ag/CS-g-VA composite sol.The effects on the preparation of Ag/CS-g-VA composite sol are studied,and the structure and thermal property of the obtained composite sol are characterized by SEM,TEM,FTIR,XRD,UV-vis and TG-DSC.The experiment results show that the optimum conditions for the preparation of Ag/CS-g-VA are as following:Vanillin concentration of 6.0%,CS-g-VA concentration of 4.0%,and silver nitrate concentration of 0.20mol/L.The inhibition of Ag/CS-g-VA composite sol against Escherichia coli and Bacillus subtilis is investigated through antibacterial tests.The results show that the antibacterial activity of Ag/CS-g-VA composite sol is more efficient than that of single CS materials.

关 键 词：天然高分子化学 壳聚糖接枝香草醛 纳米银 复合胶乳 微波辐射 抗菌性能 

分 类 号：O636[理学—高分子化学]