瑞舒伐他汀对急性心肌梗死大鼠心肌重构的作用及其机制  

Effect of rosuvastatin on myocardial remodeling and mechanism in rats with acute myocardial infarction

作　　者：吴春涛 马士新[2] 

机构地区：[1]上海市第六人民医院金山分院,上海201599 [2]上海市第六人民医院,上海200233

出　　处：《中国循证心血管医学杂志》2016年第5期560-563,共4页Chinese Journal of Evidence-Based Cardiovascular Medicine

摘　　要：目的探讨瑞舒伐他汀对急性心肌梗死(AMI)大鼠心肌重构的作用及其机制。方法选择健康成年雄性SD大鼠60只,通过结扎冠状动脉左前降支建立AMI大鼠模型51只,按照随机数表法分成假手术组、模型组和他汀组,每组各17只。他汀组大鼠灌胃给药瑞舒伐他汀钙片10 mg/(kg·d),1/日,另外两组大鼠给予等量的蒸馏水,连续给药6周。采用彩色多普勒超声诊断仪检测左室射血分数(LVEF)、左室舒张末期内径(LVEDD)及左室收缩末期内径(LVESD)。应用RT-PCR技术检测心肌组织基质金属蛋白酶-2/9/10(MMP2/9/10)、Perionstin蛋白及转化生长因子-β1(TGF-β1)m RNA表达情况。结果模型组和他汀组大鼠的LVEDD及LVESD均明显高于假手术组,LVEF显著低于假手术组,差异均具有统计学意义(P均<0.05)。与模型组相比,他汀组大鼠的LVEDD及LVESD明显下降,LVEF显著升高,差异均具有统计学意义(P均<0.05)。与假手术组相比,其余两组大鼠心肌组织中MMP-2/9/10、Perionstin蛋白及TGF-β1的m RNA表达均明显升高;其中他汀组大鼠心脏中MMP2/9/10、Perionstin蛋白及TGF-β1的m RNA表达显著低于模型组,且差异均具有统计学意义(P均<0.05)。药物干预结束后,假手术组大鼠存活率为76.47%(13/17)明显高于模型组的41.18%(7/17)和他汀组的64.71%(11/17),同时他汀组的存活率显著高于模型组,且差异均具有统计学意义(P均<0.05)。结论瑞舒伐他汀可改善AMI大鼠心脏功能,可能通过调节MMP2/9/10、Perionstin蛋白及TGF-β1的m RNA表达来调控其心肌重构。Objective To discuss the effect of rosuvastatin on myocardial remodeling and mechanism inrats with acute myocardial infarction （AMI）. Methods Male adult SD rats （n=60） were chosen and AMI modelwas established in 51 rats through ligating left coronary anterior descending branch, and then they were randomlydivided into sham-operation group, model group and rosuvastatin group （each n=17）. The rosuvastatin groupwas intragastrically given rosuvastatin calcium tablets [10 mg/（kg·d）] once a day, and other 2 groups weregiven distilled water in the same dose for 6 w. The left ventricular ejection fraction （LVEF）, left ventricular enddiastolicinner diameter （LVEDd） and left ventricular end-systolic diameter （LVESd） were detected by using colorDoppler ultrasonic diagnostic apparatus. The mRNA expressions of myocardial matrix metalloproteinase-2/9/10（MMP-2/9/10）, Perionstin protein and transforming growth factor β1 （TGF-β1） were detected by using reversetranscription polymerase chain reaction （RT-PCR）. Results LVEDd and LVESd were significantly higherand LVEF was significantly lower in model group and rosuvastatin group than those in sham-operation group（all P〈0.05）. Compared with model group, LVEDd and LVESd decreased significantly and LVEF increasedsignificantly in rosuvastatin group （all P〈0.05）. Compared with sham-operation group, the mRNA expressions ofMMP-2/9/10, Perionstin protein and TGF-β1 increased significantly in other 2 groups, and were significantlylower in rosuvastatin group than those in model group （all P〈0.05）. After drug intervention finished, the survivalrate of rat was 76.47% （13/17） in sham-operation group, which was significantly higher than that in model group[41.18% （7/17）] and rosuvastatin group [64.71% （11/17）], and meanwhile, the survival rate of rat was significantlyhigher in rosuvastatin group than that in model group （all P〈0.05）. Conclusion Rosuvastatin can improve heartfunction and control myocardial remodeling th

关 键 词：瑞舒伐他汀 急性心肌梗死 心肌重构 机制 大鼠 

分 类 号：R541.4[医药卫生—心血管疾病]