中国南方荷斯坦奶牛TRAPPC9、DGAT1、GHR、ATP1A1基因多态性与产奶性状相关性分析  

Associations of polymorphisms in TRAPPC9,DGAT1,ATP1A1 and GHR genes with milk production traits in Holstein dairy cow in Southern China

作　　者：王凯[1] 柳广斌[1] 黄特民 吴珍芳[1] 孙宝丽[1] 刘德武[1] 

机构地区：[1]华南农业大学动物科学学院/畜禽育种国家地方联合工程研究中心,广东广州510642 [2]深圳市晨光乳业有限公司,广东深圳518107

出　　处：《华南农业大学学报》2016年第4期7-12,共6页Journal of South China Agricultural University

基　　金：深圳市战略新兴产业发展专项(JSGG20141118161353890);温氏基金项目

摘　　要：【目的】研究转运蛋白颗粒复合体9(TRAPPC9)、二酰甘油酰基转移酶(DGAT1)、Na^+,K^+-ATP酶(ATP1A1)和生长激素受体(GHR)基因SNP座位多态性与我国南方荷斯坦奶牛Holstein cattle群体产奶性状的相关性。【方法】利用飞行质谱基因分型技术对SNP座位进行多态性检测,使用SAS软件进行统计分析。【结果】TRAPPC9基因rs110017379座位多态性对乳蛋白率和乳脂率有显著影响(P<0.05);DGAT1基因rs109421300座位多态性对乳脂率有显著影响(P<0.05);ATP1A1基因rs110256520座位多态性对乳蛋白率有显著影响(P<0.05);GHR基因rs41639260座位多态性对乳蛋白率有显著影响(P<0.05),对乳脂率有显著影响(P<0.05)。【结论】可以考虑将TRAPPC9、DGAT1、ATP1A1和GHR基因相关SNP应用到我国南方荷斯坦奶牛的分子标记辅助选择工作中。【Objective 】To study whether SNPs of TRAPPC9,DGAT1,ATP1A1 and GHR genes were associated with milk production traits in Holstein dairy cow in Southern China. 【Method】The Sequenom Mass ARRAY IPLEX genotyping technology was applied for SNP genotyping,and SAS software was used for statistical analysis.【Result】The association analysis showed that the rs110017379 SNP of TRAPPC9 had significant effects on protein percentage( PP) and fat percentage( FP)( P < 0. 05),the rs109421300 of DGAT1 had significant effects on FP( P < 0. 05),the rs110256520 of ATP1A1 had significant effects on PP( P < 0. 05),and the rs41639260 of GHR had significant effects on both PP and FP( P < 0. 05).【Conclusion】The SNPs of TRAPPC9,DGAT1,ATP1A1 and GHR genes can be used in molecular marker assisted selection of Holstein dairy cow in Southern China.

关 键 词：中国荷斯坦奶牛 产奶性状 基因 SNP 分子标记 

分 类 号：S813.3[农业科学—畜牧学]