外用伤科药对运动性慢性骨骼肌损伤及肌细胞IL-6表达影响研究  

Research on The Influence of External Applied Medicine to Exercise-Induced Chronic Skeletal Muscle Injuries and Muscle Cells IL-6 Expression

作　　者：牛文玉[1] 陈佑学[2] 

机构地区：[1]铜仁学院体育与健康学院,贵州铜仁554300 [2]首都体育学院,北京100191

出　　处：《体育科技文献通报》2016年第7期147-150,共4页Bulletin of Sport Science & Technology

摘　　要：目的:研究不同外用伤科药对运动性骨骼肌慢性损伤中肌细胞IL-6表达的影响以及骨骼肌细胞中IL-6在运动性慢性肌组织损伤修复中所发挥的作用。方法:采用体重2.2kg—2.5kg成年雄性新西兰兔42只,随机分为对照组、扶他林组、消痛贴组,每组21只。其中每组又分为4、5周组,每组7只。动物采用高电压低电流刺激仪刺激,使其产生主动的跑跳运动,训练方案:0.1-0.2s/次(每两次间隔12s),20min/组(两组间歇20min),3组/天,5天/周。训练结束后分别给4周、5周扶他林组和消痛贴组以不同的外用药。各组在相应的训练周(4、5)完成后取左腿腓肠肌内侧头,用4%多聚甲醛固定液保存,进行HE染色及免疫组织化学处理,光镜观察肌组织形态变化及细胞中IL-6表达的免疫组织化学图像分析,计算IL-6平均光密度值。结果:1、随着训练时间的推移,骨骼肌细胞IL-6的表达明显增多;2、外用伤科药可以降低运动性慢性骨骼肌损伤中细胞的炎症因子;3、外用伤科药可以显著降低运动性慢性骨骼肌损伤中肌细胞IL-6的表达。结论:1、骨骼肌损伤中,肌细胞的炎性因子与肌细胞IL-6的表达存在一定的联系;2、外用伤科药对慢性骨骼肌损伤中肌细胞的修复有着较明显的作用;3、运动性慢性骨骼肌损伤中,不同的外用伤科药都可以显著降低骨骼肌IL-6的表达影,二者没有显著差别。The thesis aims at discussing the influence of different external applied medicine to muscle cells IL-6 expression in exercise-induced chronic skeletal muscle injuries,as well as the role IL-6 played in exercise-induced skeletal muscleinjury rehabilitation.Methods:Randomly divide 42 adult male New Zealand rabbits weighted from 2.2kg to 2.5kg into 3 groups—control group,voltaren group and pain-relieving paste group,with7 rabbits per group.And each group is divided into 4-week group and 5-week group.High voltage and low current stimulation is used to stimulate the rabbits to run and jump actively.Training schedule:0.1-0.2s per time with 12 s intervals,20 min per set with 20 min intervals between sets,3 sets per day and 5 days a week.After training,use different medicine for voltaren group and pain-relieving paste group(both 4-week and 5-week groups).Inside muscle heads of left leg gastrocnemius muscles are sampled when the training week finished,preservedin 4% paraformaldehyde and treated with HE dyeing and immunochemistry.Then the change of organelle's tissue and immunochemical analysis on IL-6 image in cells are observed under light microscope to counts the IL-6average optical density value.Results are as follows:First,inflammatory factors are related with muscle sell IL-6 expression in skeletal muscle injuries.Second,external applied medicine is significantlyeffective for chronic skeletal muscle damage.Third,no significant differences between different external medicines in reducing skeletal sell IL-6 expression.

关 键 词：IL-6表达 外用贴药 作用 运动性肌损伤 

分 类 号：G804.2[文化科学—运动人体科学]