灵芝孢子油对人肺癌细胞的生长抑制作用及超微结构的影响  

Effect of ganoderma lucidum spore oil on growth inhibition and ultrastructure of human lung cancer cells

作　　者：袁青宁 吕高建[2] 汤莹[1] 沈亚峰[1] 雷长海[1] 杨勇骥[1] 陈新[2] 

机构地区：[1]第二军医大学基础部生物物理学教研室,上海200433 [2]华东理工大学材料科学与工程学院,上海200237

出　　处：《解剖学杂志》2016年第3期277-280,共4页Chinese Journal of Anatomy

摘　　要：目的：探讨灵芝孢子油对人肺癌细胞（A549）超微结构的影响以及对其生长的抑制作用.方法：利用CCK-8法检测灵芝孢子油对A549细胞的生长抑制作用;利用透射电镜研究灵芝孢子油对A549细胞超微结构的影响;利用乳酸脱氢酶（LDH）检测法研究灵芝孢子油对细胞膜通透性的影响;上述方法研究灵芝孢子油对体外培养的肺癌细胞A549的抑制作用.结果：CCK-8检测结果表明25 μg/ml浓度的灵芝孢子油可导致A549细胞的存活率明显下降;透射电镜结果显示,随着灵芝孢子油浓度的增加,可以观察到细胞超微结构的明显改变;LDH检测显示灵芝孢子油可使细胞的LDH释放量明显增加,且呈剂量依赖性.结论：灵芝孢子油对人肺癌细胞具有明显抑制作用.Objective:To study the effect of ganoderma lucidum spore oil on growth inhibition and ultrastructure of human lung cancer cells(A549).Methods:The effect of growth inhibition was detected by cell counting kit 8(CCK-8) method.The ultrastructure of A549 cells was observed by transmission electron microscopy.The viability of A549 cells was studied by CCK-8 test.The membrane permeability of A549 cells was studied by lactate dehydrogenase(LDH) cytotoxicity assay.Results:Ganoderma lucidum spore oil significantly decreased the viability of A549 by CCK-8 in 25μg/ml.The ultrastructure of A549 was changed and LDH release of A549 had a significant increase with a concentration-dependent relationship.Conclusion:Ganoderma lucidum spore oil can inhibit the viability of human lung cancer cells.

关 键 词：灵芝孢子油 肺癌 A549细胞 透射电镜 

分 类 号：R285[医药卫生—中药学]