HCV NS3蛋白适配子结构分析  

Structure Analysis of HCV NS3 Protein Aptamers

作　　者：黄斯瑜[1] 徐国胜[2] 

机构地区：[1]广州血液中心增城区血站,广东广州511300 [2]广州血液中心,广东广州513000

出　　处：《深圳中西医结合杂志》2016年第9期8-9,共2页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:对筛选的丙型肝炎病毒(HCV)非结构蛋白NS3适配子进行测序和结构分析。方法:使用DNAMAN V6软件对适配子序列进行多序列比对分析和最低能量二级结构分析。结果:筛选得到的6条适配体随机序列均无同源序列;寡核苷酸适配子的二级结构以茎环和凸环为主。结论:这些适配子特定的空间结构是适配子与HCV NS3蛋白特异性结合的基础。Objective The study purpose is to take sequencing and structure analysis for non-structural protein NS3 of screened HCV virus. Methods The study method is to use DNAMAN V6 software to take multiple sequence comparative analysis and minimum energy secondary structure analysis of aptamers＇ sequences. Results The study results indicate that there is no homologous sequence for random sequence of the 6 screened aptamers and the secondary structure of oligonucleotide aptamers is mainly in stem-loop and bulge loop. Conclusion Specific spatial structure of these aptamers is the basis of specific binding of aptamers and HCV NS3 protein.

关 键 词：丙型肝炎病毒 NS3蛋白 适配子 

分 类 号：R512.63[医药卫生—内科学]