穹窿主体蛋白对血管平滑肌细胞增殖的影响  

Effects of major vault protein on the proliferation of vascular smooth muscle cells

作　　者：童星[1] 江斌[1] 柏惠[1] 陈琪[1] 

机构地区：[1]南京医科大学病理生理学系,江苏南京211166

出　　处：《南京医科大学学报（自然科学版）》2016年第5期539-543,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金重点项目(81230070);973项目(2012CB517503)

摘　　要：目的 :探讨穹窿主体蛋白(major vault protein,MVP)对血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖的影响。方法:分离培养野生型小鼠VSMCs,用血小板源性生长因子(PDGF-BB)或15%胎牛血清(FBS)刺激VSMCs增殖,Western blot检测VSMCs的MVP表达水平,同时检测特异性分化标记物α-SMA和SM22α表达水平。然后用PDGF-BB刺激人主动脉平滑肌细胞(HAo SMCs),检测MVP的表达差异。再分别分离培养野生型小鼠和MVP敲除型小鼠的VSMCs,通过CCK8细胞增殖实验检测两者的增殖能力;同时用si RNA干扰的方法,使HAo SMCs中MVP低表达,检测其增殖能力的变化。结果:用PDGFBB或15%FBS刺激VSMCs后,MVP表达水平呈浓度和时间依赖性下降,α-SMA和SM22α表达水平也相应降低。MVP缺失或低表达情况下,VSMCs的增殖能力均下降。结论:MVP在VSMCs增殖中起了重要作用。Objective：To investigate the effects of major vault protein（MVP）on the proliferation of vascular smooth muscle cells（VSMCs）. Methods：The wild-type mouse VSMCs were isolated,and cultured,and the human aortic smooth muscle cells（HAo SMCs）were used. Western blot analysis was used to determine the MVP,α-SMA and SM22α. Expression of MVP stimulated by plateletderived growth factor（PDGF-BB）or 15% fetal bovine serum（FBS） was measured. MVP si RNA was used to downregulate the MVP expression in HAo SMCs. Cell proliferation CCK-8 assay was conducted to detect the proliferation ability of VSMCs in the case of MVP deficiency or downregulation. Results：After the treatment of PDGF-BB or 15% FBS,the MVP expression of VSMCs was downregulated in a time-dependent and dose-dependent manner. Compared with wild-type mouse VSMCs,the proliferation of MVP in knockout mouse VSMCs was suppressed. MVP si RNAs inhibited FBS-induced HAo VSMCs proliferation. Conclusion：MVP may played crucial effects in the proliferation of the VSMCs.

关 键 词：穹窿主体蛋白 小鼠主动脉平滑肌细胞 人主动脉平滑肌细胞 增殖 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]