海虾致死试验筛选天然活性成分的研究进展  

Research Progress on Natural Active Ingredients by the Screening Method of Brine Shrimp Lethality Test

作　　者：郭晶晶[1] 张屏[1] 李瑞娟[1] 赵红梅[1] 岳鑫[1] 包保全[1] 

机构地区：[1]内蒙古医科大学药学院,呼和浩特010059

出　　处：《世界科学技术-中医药现代化》2016年第5期889-893,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委地区项目(81160551):破痞去滞蒙药特穆日-敖日阳古抗肿瘤活性成分及其构效关系研究;负责人:包保全;内蒙古医科大学2015中青年人才团队项目(NYTD-2015004):蒙药新药创制产业创新人才团队;负责人:包保全

摘　　要：海虾致死试验是近年快速发展起来的一种天然活性成分筛选方法,广泛应用于细胞毒、抗癌和杀病原虫等药理活性的筛选,也是毒性评估的重要工具之一。该方法利用简单的生物有机体——丰年虾(Brine Shrimp,Artemia sp.)的幼虫,体外给予不同浓度的待测样品,培养一定时间后测定海虾幼虫的存活数,计算出LD50值作为活性评价指标。该方法应用整体动物进行体外实验,具有操作简单、快速、经济、结果准确、重复性好等优点。样品用量在20 mg以下,适用于天然提取物的活性筛选和活性追踪分离。The brine shrimp lethality test（BSLT）, a kind of activity screening method, that rapidly developed in recent years is widely used for screening cytotoxicity, anticancer and protozoacide natural products, as well as a major assessment of preliminary toxicities. BSLT is operated using a primitive organism nauplii of brine shrimp（Artemia sp.） which is administered by unknown samples with various concentrations. The survivals are counted and the bioactivity index LD50 is calculated after a specified period of the cultivation in vitro. BSLT is performed on intact animals with the advantages of simple steps, short period, low cost, accurate output, as well as good repeatability. Moreover, the test normally needs only a dosage of analytes less than 20 mg, which is suitable for screening and separation of bioactive natural ingredients in pharmaceutical research work.

关 键 词：海虾致死性试验 细胞毒 抗癌 杀锥虫 

分 类 号：R284[医药卫生—中药学]