CC类趋化因子受体2在人内皮细胞迁移中的作用  

The Role of C-C Motif Chemokine Receptor-2 in Human Vein Endothelial Cells Migration

作　　者：赵宁 赵俐杰 

机构地区：[1]锦州医科大学 [2]解放军95979部队卫生教研室

出　　处：《辽宁医学院学报》2016年第3期4-5,I0002,I0003,共4页Journal of Liaoning Medical University (LNMU) Bimonthly

摘　　要：目的探讨CC类趋化因子受体2(CCR2)对人脐静脉血管内皮细胞(HUVECs)迁移的作用。方法 RT-PCR和Western-blot检测单核细胞趋化蛋白-1(MCP1)对HUVECs CCR2蛋白表达的影响。Transwell实验观察CCR2拮抗剂对HUVECs迁移的影响。结果在HUVECs中CCR2蛋白表达存在MCP1浓度依赖方式,Transwell实验显示CCR2拮抗剂(RS504393)抑制细胞迁移(P<0.01)。结论 CCR2对人内皮细胞迁移有促进作用,其作用与MCP1结合相关。Objective To explore the effect of C-C motif chemokine receptor -2 （ CCR2 ） on the migration of human umbilical vein endothelial cells （HUVECs）. Methods The effect of monocyte chemoattractant protein-1 （MCP1） on the expression of CCR2 in HUVECs was measured by RT-PCR and Western blot. The effect of anti-CCR2 polyclonal antibodies （RS504393） on the migration of HUVECs was measured by Transwell migration assay. Results CCR2 was dose-dependently induced by MCP1 in HUVECs. The migrating of cells was significantly inhibited by RS504393. Conclusion CCR2 can accelerate the migration of HUVECs, which may be associated with MCP-1 binding.

关 键 词：单核细胞趋化蛋白-1 CC 类趋化因子受体2 人内皮细胞 细胞迁移 

分 类 号：Q591.2[生物学—生物化学]