粪便中短链脂肪酸检测及临床意义  

Detection of Short Chain Fatty Acids in Feces and Its Clinical Significance

作　　者：付文政[1] 张春泽[1] 贾岩峰[1] 张锡朋[1] 

机构地区：[1]天津市人民医院肛肠科,天津300121

出　　处：《中国肛肠病杂志》2016年第4期7-9,共3页Chinese Journal of Coloproctology

基　　金：天津市卫生计生委重点攻关项目.批号：2010KG206

摘　　要：运用顶空气相色谱一串联质谱法（HS-GC／MS）检测结肠癌患者与健康人群粪便短链脂肪酸，并比较两者肠道微生物代谢产物的差异性。将结肠癌患者23例设为研究组，同期体检并接受本研究的15名健康志愿者为对照组，采用HS-GC／MS检测分析2组粪便短链脂肪酸变化。结果显示，在结肠癌患者与健康人群粪便样本中均可以检测到乙酸、丙酸、丁酸、戊酸、己酸等5个直链脂肪酸以及异丁酸、异戊酸、庚酸等3个支链脂肪酸。与对照组比较，研究组粪便中的直链脂肪酸明显降低（P〈0．05），粪便中的支链脂肪酸明显升高（P〈O．05）。结果表明，HS-GC／MS检测人体肠道微生物代谢产物短链脂肪酸操作简单，灵敏度好，可作为检测结肠癌患者粪便短链脂肪酸变化的首选方法。结肠癌患者短链脂肪酸水平与健康人群的差异性可为结肠癌快速筛查及临床治疗提供理论依据。This study was using headspace gas chromatography-tandem mass spectrometry（HS-GC/MS） to detect short chain fatty acids（SCFAs） in feces of colon cancer patients and healthy population,so as to compare the diversity of metabolic product by intestinal mierooganism in above two populations; 23 patients with colon cancer were enrolled in research group,at same time 15 healthy volunteers were exami- nated physically in control group lboth groups were received detection of SCFAs,the obtained results were analyzed.As results,even in two groups there all were detected straight chain fatty acids such as hawkin- sin,propionic acid, butanoic acid, valeric acid, hexanoic acid, etc, and detected branched chain fatty acids such as isohutyric acid,isovaierie acid and enanthic acid, ere, but the straight chain fatty acids of research group had significantly reduced, and its branched chain fatty acids signficantly uplifted（ P 〈0.05,all） compared with control group.Results show that by using HS-GC/MS to detect SCFAs in human feces（i.e metabolic products by intestinal microoganisms） is of easy ＆ simple manipulation,and good sensitivity, which can be taken as the first choice for detecting SCFAs change in colon cancer patient＇s feces;And this diversi- ty of SCFAs between colon cancer patients and healthy person can provide the rational basis for fast screening and clinically treating colon cancer.

关 键 词：结肠癌 粪便 短链脂肪酸 顶空气相色谱-串联质谱法 

分 类 号：R459.3[医药卫生—治疗学]