玉米赤霉烯酮对雄性小鼠生殖系统的毒性作用  

Toxic effects of zearalenone on male reproductive system in mice

作　　者：董双[1] 何剑斌[1] 张燚[1] 龙淼[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110866

出　　处：《畜牧与兽医》2016年第6期25-29,共5页Animal Husbandry & Veterinary Medicine

基　　金：中国博士后科研基金项目(2014M551125);国家自然科学基金项目(31201961,31302152)

摘　　要：为探讨玉米赤霉烯酮(ZEA)对雄性生殖系统的毒性作用,本试验选取清洁级昆明系雄性小鼠93只,随机分成5组:25 mg/kg、50 mg/kg、75 mg/kg ZEA处理组、溶剂对照组(DMSO)、空白对照组(生理盐水),进行腹腔注射染毒,每天1次,连续5 d,于末次染毒后第2天摘眼球采血并脱颈处死动物,分离睾丸和附睾,测定脏器系数、精子密度和活力,睾丸酶的活力水平以及血清睾酮和雌激素的浓度。结果显示,与对照组相比,玉米赤霉烯酮显著地降低小鼠的体重和精液品质,睾丸乳酸脱氢酶(LDH)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)的活力随着染毒剂量的增加显著升高(P<0.01,P<0.05),血清睾酮和雌激素浓度随染毒剂量的增加显著下降(P<0.01,P<0.05)。该结果提示,玉米赤霉烯酮对雄性小鼠生殖系统具有严重的损伤作用,影响生精功能。To examine toxic effects of zearalenone （ZEA） on male reproductive system, 93 clean grade adult male Kunming mice were randomly divided into 5 groups and exposed to intraperitoneal injection of ZEA at 25, 50 and 75mg/kg body weight, DMSO and normal saline solution, respectively for 5 days. The mice were sacrified at the 6th day after eyeball blood sampling. The testis and epididymis viscera indexes, semen quality, testis enzyme activity and serum concentrations of testosterone and estrogen were assessed. The results showed that compared with the control group, ZEA significantly decreased the body weight, semen quality, increased the testis enzyme activity and decreased serum concentrations of testosterone and estrogen （P〈0. 01, P〈0. 05 ）. In conclusion, ZEA can hurt male reproductive system and affect the spermatogenic function.

关 键 词：玉米赤霉烯酮 睾丸 附睾 精液品质 

分 类 号：S856.9[农业科学—临床兽医学]