脱氢表雄酮对大鼠机体糖代谢的调节及其生化机制  

Effects of dehydroepiandrosterone( DHEA) on glycometabolism and the biochemistry mechanisms in rats

作　　者：康健[1] 陈迪[1] 王典[1] 马海田[1] 

机构地区：[1]南京农业大学动物医学院农业部动物生理生化重点实验室,江苏南京210095

出　　处：《畜牧与兽医》2016年第6期41-49,共9页Animal Husbandry & Veterinary Medicine

基　　金：江苏省自然科学基金(BK20151434)

摘　　要：为了探讨灌喂不同剂量的脱氢表雄酮(DHEA)对大鼠糖代谢的影响及生物化学其机制,采用60只雄性SD大鼠随机分为对照组、低剂量、中剂量和高剂量组,各处理组大鼠分别灌胃每天0、25、50、100mg/kg的DHEA,连续灌胃8周,测定大鼠血糖、肝糖原、肌糖原、相关激素水平、糖代谢关键酶的活性及相关基因表达量。结果表明,中剂量DHEA灌胃组大鼠的体增重相比对照组显著降低,而料重比显著升高。与对照组相比,中、高剂量的DHEA可显著增加肝糖原和肌糖原的含量,中剂量的DHEA则可显著降低血糖含量。糖代谢相关酶的活性结果表明:与对照组相比,高剂量的DHEA处理可显著提高6-磷酸果糖激酶2(PFK-2)和苹果酸脱氢酶(MDH)的活性;中剂量的DHEA处理可显著提高丙酮酸激酶(PK)和琥珀酸脱氢酶(SDH)的活性;低剂量的DHEA处理可显著提高MDH、PK和丙酮酸脱氢酶系E1的活性。糖代谢相关基因表达的结果分析表明:与对照组相比,高剂量的DHEA可显著降低磷酸烯醇式丙酮酸羧激酶(PEPCK)的mRNA表达水平,低剂量的DHEA可显著提高6-磷酸果糖激酶1(PFK1)的mRNA的表达水平。与对照组相比,低、高剂量的DHEA处理可显著提高胰岛素受体(INSR)的mRNA表达水平;低、中剂量的DHEA可显著提高胰岛素受体底物1(IRS-1)的mRNA表达水平,而中、高剂量的DHEA处理可显著提高胰岛素受体底物2(IRS-2)的mRNA表达水平。高剂量的DHEA处理可显著提高葡萄糖转运蛋白2(GLUT-2)的mRNA表达水平,而中剂量的DHEA处理可显著提高葡萄糖转运蛋白4(GLUT-4)的mRNA表达水平。说明DHEA处理主要通过促进肝脏对葡萄糖的转运、提高肝脏中糖代谢途径关键酶的活性,从而调节大鼠血糖的水平,且此种效应可能主要是能过调节胰岛素受体及胰岛素受体底物的表达而实现的。The aim of this study was to investigate the effect of DHEA on the glycometabolism in rats and its possible biochemistry mechanisms. 60 male SD rats were randomized into four groups, and the rats in each group were administrated with 0, 25, 50 and 100 mg/ （ kg · bw · d） DHEA for 8 weeks. The results showed that the addition of50 mg/ （kg · bw·d） DHEA significantly decreased body weight gain, and significantly increased ratio of feed to gain when compared to the control group. 50 and 100 rag/ （kg · bw· d） DHEA significantly increased the hepatic glycogen and muscle glycogen content, and 50 mg/ （ kg · bw · d） DHEA significantly decreased the blood sugar contents when compare to the control group. 6-phosphofructokinase-2 and malate dehydrogenase activity was markedly increased in 100 mg/ （kg· bw · d） DHEA treatment group than that in the control group. Pyruvate kinase and succinate dehydrogenase activity was significantly increased in 50 mg/ （kg · bw · d） DHEA treatment group. Compared to the control group, the pyruvate dehydrogenase complex E1, pyruvate kinase and malate dehydrogenase activities were markedly increased in 25 mg/ （ kg · bw · d） DHEA treatment group. Insulin receptor （INSR） mRNA level was markedly increased in 25 and 100 mg/ （ kg · bw · d） DHEA treatment group than that in the control group. Com- pared to the control group, the insulin receptor substrate-1 mRNA level was dramatically increased in 25 and 50 mg/ （kg · bw · d） DHEA treatment group, while the insulin receptor substrate-2 mRNA level was markedly increased in 50 and 100 mg,/ （kg ·bw · d） DHEA treatment group. In addition, glucose transporter-4 mRNA level was significantly increased in 50 rag/ （kg · bw· d） DHEA treatment group,and the glucose transporter-2 mRNA level was significantly increased in 100 mg/ （kg ·bw · d） DHEA treatment group. It suggests that DHEA regulated the blood glucose level via promoting glucose transportation and regulating the activates of key

关 键 词：脱氢表雄酮 糖代谢 生化机制 大鼠 

分 类 号：S831.5[农业科学—畜牧学]