检测外环境霍乱弧菌的方法探讨  

Exploration on the detection mathods of Vibrio cholerae from external environmental

作　　者：谢晓红[1] 王洁[1] 蔡卫红[1] 王仁刚[1] 陶力新 

机构地区：[1]上海市奉贤区疾病预防控制中心,上海201499

出　　处：《中国卫生检验杂志》2016年第11期1600-1601,1606,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的提高外环境霍乱弧菌的阳性检出率。方法通过荧光定量PCR技术、免疫磁珠分离技术和常规分离培养技术相结合的综合检测方法,与荧光定量PCR技术结合常规分离培养技术和单纯的常规分离培养技术的检测方法进行比较,观察分析其阳性检出率。结果荧光定量PCR技术、免疫磁珠分离技术和常规分离培养技术相结合的综合检测方法,霍乱弧菌阳性检出率(1.05%)明显高于荧光定量PCR技术结合常规分离培养技术的阳性检出率(0.53%)和单纯的常规检测技术的阳性检出率(0.26%)。结论将PCR方法作为筛查的基础,进行免疫磁珠分离技术结合常规分离培养技术应用于霍乱弧菌的检测,可降低工作量,提高工作效率和工作质量,明显地提高阳性检出率。Objective To improve the detection rate of positive Vibrio cholerae from environment. Methods To compare the combined detection by fluorescence quantitative PCR and immunomagnetic separation technique and the conventional separation cultivation with the combined detection by the fluorescence quantitative PCR and conventional isolation and cultivation technology and simple routine isolation and culture techniques,to observe and analyze the positive detection rate. Results The results showed that the positive rate（ 1. 05%） of detection of Vibrio cholerae by qRT-PCR / IMS / EC method was significantly higher than by the qRT-PCR / EC method（ 0. 53%） and EC method（ 0. 26%）. Conclusion The PCR was as the basis of screening,applying the immunomagnetic separation technology combined with conventional isolation and culture technique to the detection of Vibrio cholerae,which could reduce workload and improve the efficiency and quality of work,and can obviously improve the positive detection rate.

关 键 词：外环境 霍乱弧菌 检测方法 阳性率 

分 类 号：R115[医药卫生—公共卫生与预防医学]