多尼培南抗铜绿假单胞菌生物被膜的体外活性研究  

In vitro activities of doripenem against Pseudomonas aeruginosa biofilm

作　　者：王素霞[1] 陈向东[1] 汪辉[1] 谢少朵 魏洪璇[1] 潘云[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009

出　　处：《中国抗生素杂志》2016年第6期466-472,共7页Chinese Journal of Antibiotics

摘　　要：目的探究新型碳青霉烯类抗生素多尼培南(doripenem,DOR)抗临床铜绿假单胞菌生物被膜的活性并进一步探究亚抑菌浓度多尼培南抑制生物被膜的作用机制。方法采用微孔板法建立临床铜绿假单胞菌生物被膜体外模型。微量肉汤稀释法测定多尼培南对铜绿假单胞菌的最低抑菌浓度(minimal inhibitory concentration,MIC)和最低抑制生物被膜浓度(minimal biofi lm inhibitory concentration,MBIC)。结晶紫染色法测定生物被膜的形成和黏附,活菌计数法测定生物被膜内菌数。平板分析法测定运动性,微生物碳氢吸附能力(microbial adhesion to hydrocarbons,MATH)测定评估细胞疏水性。结果多尼培南具有较强的抗铜绿假单胞菌浮游菌和生物被膜菌活性,MIC50为0.5μg/m L,MBIC50为2μg/m L。明显抑制生物被膜的形成和减少已形成生物被膜的量,降低铜绿假单胞菌的运动性和疏水性。结论多尼培南能有效抑制铜绿假单胞菌生物被膜,有望用于相关感染的治疗。Objective To study the activity and the mechanism of doripenem, a novel carbapenem, against clinical Pseudomonas aeruginosa biofilm. Methods Biofilm model of clinical P aeruginosa was established using the 96-well microtiter plate. Minimal inhibitory concentration （MIC） and minimum biofilm inhibitory concentration （MBIC） were determined by the broth microdilution technique. Biofilm formation and adhesion were assayed using a modified crystal violet staining method. The number of living bacteria in biofilm was counted by viable count. The motility was measured on agar plates. Cell surface hydrophobicity was assessed by microbial adhesion to hydrocarbons. Results Doripenem appears to be a potent agent which can inhibit the biofilm formation and destruct pre-formed biofilm. The MIC50 is 0.5μg/mL, MBIC50 is 2μg/mL. It repressed motility and cell surface hydrophobicity of P aeruginosa. Conclusion Doripenem is effective to inhibit P. aeruginosa biofilm and promising to treat for biofilm-related infections.

关 键 词：铜绿假单胞菌 生物被膜 多尼培南 

分 类 号：R978.1[医药卫生—药品]