外周血中CD19^+ CD5^+ B细胞和白细胞介素-10对系统性红斑狼疮患者病情的影响  

Expression and significance of CD19^+ CD5^+ B cells and IL-10 in patients with systemic lupus erythematosus

作　　者：鄂维建 赵玲莉[1] 李玉玲[1] 

机构地区：[1]青海大学附属医院检验科,青海西宁810001

出　　处：《中国现代医学杂志》2016年第11期59-62,共4页China Journal of Modern Medicine

摘　　要：目的探讨外周血中CD19^+CD5^+B细胞和白细胞介素-10(IL-10)对系统性红斑狼疮(SLE)患者病情发生、发展的影响,为其临床研究提供参考依据。方法选择65例稳定期SLE患者及65例活动期SLE患者,同时选择70例健康人群作为对照组,采用流式细胞术检测外周血中CD19^+CD5^+B细胞数量,采用酶联免疫吸附法检测IL-10水平。比较3组的差异,并分析上述指标与SLE活动度评分(SLEDAI)及补体的相关性。结果活动组及稳定组CD19^+CD5^+B细胞数量、白蛋白、C3、C4均低于对照组,IL-10、红细胞沉降率(ESR)高于对照组,差异有统计学意义(P<0.05),活动组CD19^+CD5^+B细胞数量、白蛋白、C3、C4低于稳定组,SLEDAI评分、IL-10、ESR高于稳定组,差异有统计学意义(P<0.05)。CD19^+CD5^+B细胞数量与SLEDAI评分呈负相关,IL-10与SLEDAI评分呈正相关(P<0.05)。随着SLEDAI评分的增加,CD19^+CD5^+B细胞数量呈下降趋势,而IL-10水平呈上升趋势,差异有统计学意义(P<0.05)。结论 SLE患者外周血CD19^+CD5^+B细胞数量降低,而IL-10水平升高,随着病情活动程度的增加,外周血CD19^+CD5^+B细胞及IL-10水平变化更明显,可以作为评价SLE活动性的指标。Objective To study the expressions of CD19^＋ CD5^＋ B cells and interleukin 10 （IL-10） and their clinical significance in patients with systemic lupus erythematosus （SLE）. Methods In this study 65 SLE-stable patients, 65 SLE-active patients and 70 healthy control people were included. CD19＋ CD5＋ B cell subsets were detected with flow cytometry. IL-10 level in serum was detected with enzyme linked immunosorbent assay （ELISA）. The correlations of CD19^＋ CD5^＋ B cell count and serum IL-10 level with SLE disease activity index （SLEDAI） score and complement were analyzed. Results The CD19^＋ CD5^＋ B cell count, albumin, C3 and C4 in the SLE-active group and SLE-stable group were significantly lower than those in the control group （P 〈 0.05）. The IL-10 and ESR in the SLE-active and stable groups were higher than those in the control group, the differences were statistically significant （P 〈 0.05）. The CD19^＋ CD5^＋ B cell count, albumin, C3 and C4 in the SLE-active group were significantly lower than those in the SLE-stable group （P 〈 0.05）. The SLEDAI score, IL-10 and ESR in the SLE-active group were higher than those in the SLE-stable group, the differences were statistically significant （P 〈 0.05）. The CD19＋ CD5＋ B cell count was negatively related with the SLEDAI score, but IL-10 was positively related with the SLEDAI score （P 〈 0.05）. As the SLEDAI score increased, CD19^＋ CD5^＋ B cell count showed a lowering trend, and IL-10 showed an uptrend.Conclusions CD19＋ CD5＋ B cells and IL-10 may participate in the occurrence and development of SLE. The changes of CD19^＋ CD5^＋ B cells and IL-10 in the peripheral blood might contribute to the pathogenesis and correlate with the disease activity of SLE, which can be used as evaluation indexes of SLE activity.

关 键 词：B细胞 白细胞介素-10 系统性红斑狼疮 

分 类 号：R593.24[医药卫生—内科学]