鳞翅目昆虫Cry毒素受体与人同名蛋白的生物信息学分析  

Bioinformatics analysis of relationship between Cry toxin receptor of lepidopteran insects and the same name protein of Homo sapiens

作　　者：陈贵东[1,2] 李燕芳[1] 何颖[1] 邹泽红[1] 陶爱林[1] 

机构地区：[1]广州医科大学附属第二医院,广东省过敏反应与免疫重点实验室/变态反应国家临床重点专科/呼吸疾病国家重点实验室变态反应研究室,广东广州510260 [2]广州医科大学附属第四医院病理科,广东广州511447

出　　处：《生物技术》2016年第3期272-279,共8页Biotechnology

基　　金：国家科技重大专项重大课题(“转基因生物的食用和饲用安全评价技术”;No.2014ZX08011-005);国家科技重大专项重点课题(“改善过敏原性评价方法的对比物的创建研究”;No.2014ZX0801105B);广东高校科技创新项目(“优良待转化基因编码蛋白过敏原性医学评估与改造研究”;No.2013KJCX148)

摘　　要：[目的]生物信息学分析鳞翅目昆虫Cry毒素受体氨肽酶N(APN)、类钙黏蛋白(Ca LP)、碱性磷酸酶(ALP)与人同名蛋白的关系。[方法]NCBI数据库获取人和鳞翅目昆虫的APN、Ca LP、ALP的氨基酸序列,利用生物信息学软件对序列一致性、系统发育关系、结构域、三维结构相似性等进行预测和分析。[结果]APN系统发育关系最近的是人与家蚕,两者序列一致性最高58.06%,含2个相似结构域,三维结构相似性显著(62.80%,P<0.05)。ALP系统发育关系最近的是人与家蚕,两者序列一致性最高46.03%,含2个相似结构域,三维结构相似性显著(59.63%和59.59%,P<0.05)。人与鳞翅目昆虫的Ca LP系统发育关系较远;Ca LP序列一致性最高是人与棉铃虫(28.12%),两者含2个相似结构域,三维结构相似性不显著(18.75%,P>0.05)。[结论]人和家蚕两者间的APN、ALP系统发育关系最近,序列一致性最高,含有相似的结构域且三维结构相似性显著,可能具有相似的生物学功能。[ Objective ] To analyze the relationship between cry toxin receptor aminopeptidase - N （ APN）, cadherin - like pro- tein （CaLP）, alkaline -phosphatase （ALP） of lepidopteran insects and the same name proteins of Homo sapiens by bioinforma- tics. [ Methods ] The amino acid sequences of APN, CaLP, ALP of Homo sapiens and lepidopteran insects were acquired from NCBI database. All sequences were predicted and analyzed by bioinformatics software in the following aspects, such as sequence identity, phylogenetic trees, protein domains and the similarity of three -dimensional structure. [ Results ] There was the closest phylogenetic relationship of APN between Homo sapiens and Bombyx mori. Their APN sequence identity was the highest （58. 06% ） , and their three - dimensional structure similarity was significant （62.80% , P 〈 0.05 ） , containing two similar domains. There was the closest phylogenetic relationship of ALP between Homo sapiens and Bombyx mori. Their ALP sequence identity was the highest （46.03 % ） , and their three -dimensional structure similarity was significant （59.63% and 59.59% , P 〈 0. 05）, containing two similar domains. The phylogenetic relationship of CaLP between Homo sapiens and lepidopteran insects was far. The CaLP sequence identity between Homo sapiens and Helicoverpa armigera was the highest （28.12%）. And their three - dimensional structure similarity was not significant （ 18.75% ,P 〉 0.05 ）, containing two similar domains. [ Conclusion] The phylogenetic relationship of both APN and ALP between Homo sapiens and Bombyx mori was closest. Their sequence identity was highest. Their three - dimensional structure was significant similary, containing similar domains. It can be conjectured that they may have similar biological functions.

关 键 词：鳞翅目昆虫 Cry毒素受体 氨肽酶N 类钙黏蛋白 碱性磷酸酶 生物信息学 

分 类 号：Q811.4[生物学—生物工程]