L-精氨酸生物合成机制及其代谢工程育种研究进展  

Progress in biosynthesis and metabolic engineering of L-Arginine producer

作　　者：程功[1] 徐建中[1] 张伟国[1] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《微生物学通报》2016年第6期1379-1387,共9页Microbiology China

基　　金：国家高技术研究发展计划项目(863计划)(No.2008AA02Z212)~~

摘　　要：L-精氨酸是人体半必需的氨基酸,在生命代谢过程中起着非常重要的作用,且具有广泛的应用价值及市场需求。目前,L-精氨酸主要采用微生物发酵法进行生产,为了提高L-精氨酸的产量和稳定性,最有效的方法是优化L-精氨酸生产菌株,通过代谢工程改造微生物菌株有望达到这一目的。本文分析了微生物中L-精氨酸的代谢途径和调控机制,并综述了构建高产L-精氨酸的代谢工程策略。此外,展望了菌株稳定性和底物扩展利用的未来研究方向。L-Arginine, a semi-essential amino acid, plays an important role in the metabolism of human life. It has a wide and expanding application in industry with a fast growing market demand. Currently, the main method of production of L-Arginine is microbial fermentation. To develop microbial strains with high productivity and stability, the most effective method is to optimize the L-Arginine producing strains. And fortunately, metabolic engineering method can be used to screen the efficient L-Arginine producing strains, and has obtained the certain success. In this mini-review, the metabolic pathway and regulatory mechanism of L-Arginine biosynthesis in microorganisms were analyzed, and the strategies for metabolic engineering the efficient L-Arginine producing strains were summarized.Furthermore, the prospects of future research about the stability of L-Arginine engineering strains and the expansions of substrate utilization were discussed.

关 键 词：L-精氨酸 代谢工程 生物合成途径 调控机制 

分 类 号：Q936[生物学—微生物学]