采收及加工方法对金边蚂蟥抗凝血酶活性影响的研究  

Effects of harvest and different processing methods on anti-thrombin activity of Poecilobdella manillensis

作　　者：勾玲[1] 程搏幸[1] 郭巧生[1] 史红专[1] 刘飞[1] 卢昱希 王嘉[1] 闫士猛 吴曼郡 戴道新[1] 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095

出　　处：《中国中药杂志》2016年第11期2087-2092,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81473306;81503186);中国博士后科学基金面上项目(2014M551614);江苏省博士后科研项目(1302032C)

摘　　要：研究了采收及不同加工方法对金边蚂蟥抗凝血酶活性的影响规律。分别设置了6种加工方法,4个部位和5个体重等级,采用凝血酶直接滴定法分别对各处理样品进行抗凝血酶活性测定。结果表明,在加工方法中,金边蚂蟥鲜品经冷冻干燥处理后其抗凝血酶活性最高,达1 303.56 U·g^(-1),而90℃烘干样品最低,为15.44 U·g^(-1);在不同部位中,金边蚂蟥头部的抗凝血酶活性最高,为226.42 U·g^(-1),尾部仅为102.12 U·g^(-1);在不同体重级别中,金边蚂蟥体重≤10 g时,抗凝血酶活性最高,为328.86 U·g^(-1),体重≥40 g时,抗凝血酶活性最低,为87.71 U·g^(-1)。综上所述,采收及不同加工方法对金边蚂蟥抗凝血酶活性均有显著性影响。在金边蚂蟥加工中应该选取体质量在20~30 g的金边蚂蟥,优先选用超低温冷冻干燥方法进行干燥,其次选用晒干方法进行干燥。The effects of harvest and different processing methods on the anti-thrombin activity of Poecilobdella manillensis were respectively studied. The indicators included processing methods （ vacuum freeze drying, fresh homogenate, drying under sunlight, freezing, scalding, baking under different temperatures）, different parts （entire body, cephalon, pygidium, exudate） and body weights （≤ 10, 10-20, 20-30, 30-40, ≥140 g）. The anti-thrombin activities of P. manillensis with different processing methods were evaluated by direct anti-thrombin titration. The results indicated that the processing methods significantly affected the anti-thrombin activities of P. manillensis. Among the 11 groups, the anti-thrombin activity of P. manillensis processed with vacuum freeze drying （ 1 303. 56 U . g^-1 ） was significantly highest than the other groups （P 〈0. 05）, and that processed with baking under 90 ℃ （ 15.44 U . g^-1 ） was the lowest. The anti-thrombin activity of the cephalon of P. maniUensis （226. 42 U . g^-1 ） was the highest, and that of the pygidium （102. 12 U . g^-1） was lowest; the anti-thrombin activities for different body weights were significantly different （P 〈0. 05） ; and among the five groups, the body weight of ≤10 g （ 328. 86 U . g^-1） was the highest （ P 〈 0.05 ）, and the body weight of ≥ 40 g （87. 71 U . g^-1 ） was the lowest. In conclusion, harvest and different processing methods had a significant impact on the anti-thrombin activities of P. manillensis. In the study, for the optimal processing method for P. manillensis, the body weight between 20-30 g is recommended, and the vacuum freeze drying is preferred, which is followed by the drying under sunlight.

关 键 词：金边蚂蟥 采收 加工方法 抗凝血酶活性 

分 类 号：R283[医药卫生—中药学]