大鼠骨髓间充质干细胞三种移植途径在急性肾梗阻模型中的归巢研究  

Study on homing of bone marrow - derived mesenchymal stem cells transplanted by three routes in rat acute renal obstruction model

作　　者：李金东[1] 刘振湘[1] 邓湘东[2] 杨振[1] 曹卉[2] 程庆[1] 金宏庭[1] 左亚丽[1] 李东辉[1] 白志明[1] 

机构地区：[1]中南大学湘雅医学院附属海口市人民医院泌尿外科,海口570208 [2]中南大学湘雅医学院附属海口市人民医院中心实验室,海口570208

出　　处：《中华实验外科杂志》2016年第6期1480-1484,共5页Chinese Journal of Experimental Surgery

基　　金：海口市重点科技项目（2012-071）

摘　　要：目的对。肾动脉、尾静脉、包膜下3种间充质干细胞（MSCs）移植方式的归巢率。方法比较通过可复性左侧输尿管梗阻的大鼠模型（R—UUO）模拟临床急性肾梗阻的病变。4周龄雌性sD大鼠，无特定病原体（SPF）级，30只，随机数字表法分为3组，每组每个时间点各5只，采用经肾动脉、尾静脉、包膜下3种方式移植荧光标记的骨髓间充质干细胞[1，1’-双十八烷基-3，3，3’，3’-四甲基羰花青碘化物（DiR）．BMSCs]，结合小动物活体成像系统（IVIS）对移植后1、48h细胞在体内的归巢进行观察及分析。结果MSCs不同移植方式在梗阻侧肾脏的早期归巢率分别为：肾动脉组为（9．59×10^5±5．26×10^5）／g，包膜下组为（17．65×10^5±4．77×10^5）／g，尾静脉组为（2．18×10^5±0．45×10^5）／g，3组比较差异有统计学意义（P〈0．05）。结论3种移植途径的早期移植效率由高到低依次为包膜下移植、肾动脉移植、尾静脉移植。实际应用中，要根据具体情况选择合理的移植方式。Objective To evaluate and compare the homing of MSCs （Mesenchymal Stem Cells） transplanted through three routes： renal artery, tail vein and subcapsular. Methods It used an animal model of reversible unilateral ureteral obstruction （ R - UUO） to simulate clinical acute renal obstruction. Thirty SPF femal SD rats （4w） were divided into three groups. Every group contained 5 rats at each time point; MSCs labeled with 1, 1＇ - dioctadecyl - 3, 3, 3＇, 3＇ - tetramethylindotricarbocyanine iodide （DiR） were transplanted into thereversible unilateral ureteral obstruction （R- UUO） animals through three routes： re- nal artery, tail vein and subeapsular. Homing of MSCs was evaluated by in vivo imaging system （IVIS）. Re- sults The homing efficiency of the three delivery routes is： renal artery （9. 59 ×10^5±5. 26 ×10^5） cells/g, subcapsular （17.65×10^5±4.77×10^5） cells/g, tail vein （2. 18×10^5±0.45×10^5） cells/g. It was significantly different at the early stage （P 〈 0. 05）. Conclusion The homing efficiency of MSCs transplanted through the three routes is： subcapsular, renal artery, tail vein, from high to low. Although the transplantation effi- ciency of the three delivery routes was the same as the results of MSCs homing efficiency, it need to choose a reasonable route according to actual condition in practical application.

关 键 词：间充质干细胞 可复性单侧输尿管梗阻 活体成像系统 移植途径 归巢 

分 类 号：R692[医药卫生—泌尿科学] R-332[医药卫生—外科学]